在3T3-L1脂肪细胞诱导分化中UCP4基因表达水平的变化及TNF-α对其调节作用的研究  

Expression of UCP4 gene during differentiation of 3T3-L1 preadipocytes and regulative role of TNF-α

作　　者：周旭华[1] 郭锡熔[2] 杜娟[1] 倪毓辉[2] 潘晓勤[2] 陈荣华[2] 

机构地区：[1]江苏省连云港市第二人民医院儿科,222023 [2]南京医科大学儿科医学研究所

出　　处：《中华内分泌代谢杂志》2006年第4期378-380,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目(30371502);江苏省自然科学基金资助项目(BK2001120);江苏省卫生厅资助项目(H200362);2002年江苏省高校青蓝工程资助项目(200262);2004年连云港市科技局社会发展项目(SH0412)

摘　　要：观察3T3-L1脂肪细胞诱导分化过程中UCP4基因mRNA表达水平的变化,探讨TNF-α对成熟脂肪细胞中UCP4基因表达水平的调节作用。研究发现UCP4基因在3T3-L1细胞分化过程中表达逐渐下调,其机制可能有利于脂肪细胞的分化成熟和脂质积聚,与肥胖发生有关;TNF-α能够上调成熟脂肪细胞中UCP4基因的表达,其效应总体趋势上呈现时间反应性特征。The change of UCP4 gene expression during differentiation of 3T3-L1 preadipocyte was observed and the regulative role of tumor necrosis factor-α （TNF-α） in UCP4 gene expression in mature adipocytes was explored. The results showed that down-regulation of UCP4 gene mRNA expression during differentiation of 3T3-L1 preadipocyte may contribute to the differentiation, maturing and adipogenesis of adipocytes and that UCP4 gene may be related to the etiology of obesity. TNF-α can upregulate the mRNA expression of UCP4 gene in the mature adipocytes. The effect of TNF-α on UCP4 gene expression is generally of time-responsive correlation.

关 键 词：解偶联蛋白4基因 脂肪细胞分化 肿瘤坏死因子Α 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]