急性碘过量时FRTL细胞钠碘转运体表达的变化  

Changes of sodium iodide symporter expression in FRTL cells during acute iodine excess

作　　者：聂秀玲[1] 孙云[1] 李兰英[1] 陈祖培[1] 

机构地区：[1]天津医科大学内分泌研究所,300070

出　　处：《中华内分泌代谢杂志》2006年第4期386-387,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金重点资助项目(30230330)

摘　　要：FRTL细胞分别在含有10-6-10-3mol／L碘化钾的培养基中培养24、48和72 h,采用RT- PCR和Western印迹,检测钠碘转运体(NIS)mRNA和蛋白水平的变化。FRTL细胞在10-6-10-3mol／L高碘培养基中培养24、48 h,NIS mRNA水平与对照组相比。差异无统计学意义。而FRTL细胞在10-6-10-3 mol／L高碘培养基中培养48、72 h,NIS蛋白呈逐渐下降趋势,碘浓度越高,NIS蛋白下降越明显。本研究显示急性碘过量不影响NIS mRNA的表达,但可下调NIS蛋白,碘过量通过转录后水平调节NIS的基因表达。FRTL cells were incubated in the culture medium containing 10^-6～10^-3mol/L KI for 24, 48 and 72 h, and the levels of sodium iodide symporter （NIS） mRNA and protein were measured using RT-PCR and Western blot. The levels of NIS mRNA in FRTL cells incubated with different concentrations of iodide for 24 and 48 h showed no significant difference as compared with the control group, however, NIS protein was reduced gradually in FRTL cells incubated with different concentrations of iodide for 48 and 72 h as compared with that in control group. The higher the iodide concentration, the lower the levels of NIS protein. The results show that acute iodine excess does not affect the expression of NIS mRNA, but down-regulates NIS protein expression. Iodine excess may regulate the expression of NIS through post-transcription.

关 键 词：FRTL细胞 碘过量 钠碘转运体 

分 类 号：R581[医药卫生—内分泌]