17种中药提取物对6种牙周可疑致病菌的体外抑菌实验  

Inhibition of extracts from 17 Chinese herbs on periodontal pathogenic microbes

作　　者：朱彩莲[1] 李鸣宇[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院上海市口腔医学研究所,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2006年第4期434-436,共3页Shanghai Journal of Stomatology

摘　　要：目的:评价17种中药提取物对6种牙周可疑致病菌的体外抑菌效果。方法:选择17种有较强抑菌作用的中草药提取物,采用微量液体稀释法,分别对牙龈卟啉单胞菌ATCC33277、具核梭杆菌ATCC25586、伴放线放线杆菌ATCCY4、产黑普氏菌ATCC25261、黏性放线菌ATCC19246、内氏放线菌ATCC45进行体外抑菌实验。结果:丹酚酸B、厚朴酚对产黑普氏菌、具核梭杆菌的MIC值在0.053～0.313mg/ml之间,抑菌效果最强;其次为川穹嗪、苦参碱、甘草酸、龙胆苦甙、苦豆碱、黄芩甙、齐敦果酸、银杏叶的MIC值在0.106～0.625mg/ml之间;白芍总苷、茴香醛、大黄素、广霍香、盐酸小檗碱、连翘苷、葛根素抑菌效果最差。结论:在本研究选择的17种中药提取物中,丹酚酸B、厚朴酚体外抑菌效果最好。PURPOSE： To evaluate the efficiency of 17 Chinese herbs on periodontal pathogenic microbes. METHODS： 17 efficient substances from Chinese herbs were purchased from Chinese Drug Identification Bureau, including magnesium lithospermate B, magnolol, tetramethyl pyrazine, matrine, dycyrrhizin, gentiopicrin, aloperin, baicalin, oleanolic acid, ginkgo seed, total glucosides of paeony capsules, anisldehyde, archin, cablin patchouli, hydrochloric acid Berberine, forsythin, and kakonein. Antimicrobial sensitivity tests of broth microdilution methods on 96-microwell plate were carried out for identification of the antimicrobial activity of extracts against six species of microorganisms： Actinobacillus actinomycete mitans（Aa） Y4, Actinomycetes viscosus（Av） 19246, Porphyromonas gingivalis（Pg） 33277, Fusobacterium necrophorum（Fn） 25286, Actinomyces naeslundii（An） wvl 45 and Prevotella nigrescens（Pn）. RESULTS： It was found that magnesium lithospermate B and magnolol showed the most efficient inhibition on microorganism of Pn and Fn, with the MIC being 0.053 and 0.313mg/ml for Pn and Fn,respectively. Tetramethyl pyrazine, matrine, dycyrrhizin, gentiopicrin, aloperin, baicalin, and oleanolic acid had better inhibition than total glucosides of paeony capsules, anisldehyde, archin, cablin patchouli, hydrochloric acid berberine, forsythin, and kakonein. CONCLUSION： The Chinese herbs, magnesium lithospermate B and magnolol are efficient agents for inhibition against periodontal pathgenic microbes.

关 键 词：中药提取物 牙周病 致病菌 

分 类 号：R285.5[医药卫生—中药学]