介导豚鼠腹腔神经节细胞5-羟色胺去极化反应的受体亚型  

5-HT receptor subtype-mediated depolarization in guinea pig celiac ganglion neurons

作　　者：蔡圣荣[1] 黄振信[2] 

机构地区：[1]安徽中医学院生理学教研室,安徽合肥230038 [2]安徽医科大学生理学教研室,安徽合肥230032

出　　处：《中国药理学通报》2006年第7期865-869,共5页Chinese Pharmacological Bulletin

摘　　要：目的在豚鼠腹腔神经节(CG)细胞上鉴定介导5-羟色胺(5-HT)去极化反应的受体亚型。方法应用离体细胞内记录技术。结果赛庚啶(cyproheptad ine,5-HT1/2受体拮抗剂)和BRL 24924(5-HT1P受体拮抗剂)可逆地阻抑5-HT慢去极化反应,而sp iperone(5-HT1A受体拮抗剂),和m ian-serin(5-HT2受体拮抗剂),对5-HT慢去极化反应无明显影响;MDL 72222(5-HT3受体拮抗剂)对5-HT慢去极化反应无明显影响,却能够可逆地阻抑5-HT快去极化反应;压力注射MCPP(5-HT1P受体激动剂)可使5-HT敏感的CG神经元出现慢去极化反应且此反应能够被BRL 24924可逆地阻抑。结论豚鼠CG细胞的5-HT快、慢去极化反应分别由5-HT3和5-HT1P受体介导的。Aim To identify the serotonin（5-HT）subtype receptor mediated 5-HT-induced depolarization in guinea pig celiac ganglion （CG） neurons. Methods Intracellular recordings were made from the isolated guinea pig CG neurons. Results Cyproheptadine （ 5- HT1/2 antagonist） and BRL 24924 （5-HTIP receptor antagonist） reversibly inhibited 5-HT-induced slow depolarization. Whereas, spiperone（5-HTiAreceptor antagonist） and mianserin （5-HT2 receptor antagonist ） could not inhibit 5-HT-induced slow depolarization.MDL 72222 （5-HT3 receptor antagonist） could not inhibit 5-HT-induced slow depolarization, but it could reversibly inhibit 5-HT-induced fast depolarization. Pressure ejection of MCPP （5-HT1P receptor agonist） on 5-HT sensitive neurons could induce a slow depolarization which could be inhibited by BRL 24924. Conclusion 5-HT-induced fast and slow depolarization is mediated by 5-HT3 and 5-HT1P receptor, respectively.

关 键 词：5-羟色胺 受体 腹腔神经节 激动剂 拮抗剂 

分 类 号：R332[医药卫生—人体生理学] R322.85[医药卫生—基础医学]