经腹部诊断性超声对人早孕绒毛组织的影响  

Effects of diagnostic transabdominal ultrasound on human villi

作　　者：夏飞[1] 李子军[1] 何军[1] 沈宗姬[1] 

机构地区：[1]苏州大学附属第一医院妇产科,江苏苏州215006

出　　处：《中国医师杂志》2006年第9期1153-1156,共4页Journal of Chinese Physician

基　　金：江苏省卫生厅科技发展基金(H200324)

摘　　要：目的通过诊断性超声对人早孕绒毛组织超微结构及凋亡相关蛋白的研究,探讨诊断性超声对人早孕绒毛组织的安全性问题。方法选取健康早孕而自愿终止妊娠的妇女35例,随机分组,不同时间的经腹部诊断性超声波作用后行人工流产取绒毛组织,透射电镜观察其超微结构,同时半定量RT-PCR分析绒毛组织中Caspase-3 mRNA和L ivin mRNA的表达。结果5 m in组与正常组比较无明显的超微结构变化,而15 m in组与正常组比较呈现较明显的变化。同时在不同辐照时间下Caspase-3 mRNA的表达,10 m in组和15 m in组与正常组比较差异有统计学意义(P<0.05);L ivinαmRNA的表达与正常组比较差异有统计学意义(P<0.05);L ivinβmRNA的表达与正常组比较差异无统计学意义(P>0.05)。结论常规剂量的经腹部诊断性超声对早孕绒毛组织是安全的。Objective To explore the security of diagnostic transabdominal ultrasound on human villi. Methods Thirty-five healthy early pregnant women who had accepted labor abortion were randomly divided into different groups. The villi were taken immediately after the ultrasound irradiation and labor abortion. The uhrastructure of human villi was observed by transmission electron microscope （TEM） and the expressions of Caspase-3 mRNA and Livin mRNA were analyzed by semi-quantitative RT-PCR. Results Compared with normal group, no significant uhrastructural changes were observed in 5 minutes group, and changes in 15 minutes group showed great difference. The expression of Caspase-3 mRNA and LivinotmRNA in 10 minutes group and 15 minutes group showed great significance compared with that of normal group （ P 〈0.05）. The expression of LivinotmRNA showed great significance in 10 minutes group and 15 minutes group （ P 〈0. 05） and LivinβmRNA shows no great significance （ P 〉 0. 05 ）. Conclusion The conventional acoustic exposure of diagnostic transabdominal ultrasound is safe for human villi.

关 键 词：超声检查/副作用 妊娠初期 绒毛膜绒毛 

分 类 号：R445.1[医药卫生—影像医学与核医学] R715[医药卫生—诊断学]