尿液流式细胞分析仪红细胞激光参数与相差显微镜尿红细胞形态学相关性分析  

Relationship analysis of urine RBC morphology between UF-100 and phase contrast microscope

作　　者：夏运成[1] 张旭光[1] 李志兰[1] 许向青[1] 姜文玲[1] 李江[1] 

机构地区：[1]中南大学湘雅二医院肾病研究所肾内科,湖南长沙410011

出　　处：《中国医师杂志》2006年第9期1181-1183,共3页Journal of Chinese Physician

摘　　要：目的研究尿液流式细胞分析仪RBC激光参数与相差显微镜尿红细胞形态学的相关性。方法对500份新鲜尿标本,采用日本UF-100流式细胞分析仪分析RBC激光参数与相差显微镜尿红细胞形态学之间的关系。结果观察相差显微镜与流式细胞分析仪RBC-info尿红细胞均一型、变异型、混合型形态符合率分别为91.4%、94.4%、83.3%,与RBC-70Fsc符合率分别是94.9%、95.7%、94.7%,与RBC-Fsc-DW符合率分别是84.4%、86.8%、90.5%,流式细胞分析仪与相差显微镜的特异性在肾小球性血尿和非肾小球性血尿分别是84.3%、88.1%和83.3%、87.9%。结论UF-100测定RBC-info、RBC-P70Fsc及RBC-Fsc-DW参数与相差显微镜镜尿红细胞形态学均高度相关,显示全自动UF-100流式细胞分析仪具有操作简便、快速、准确性高等优点,对临床肾脏疾病的诊断、治疗以及预后判断具有指导意义,值得推广应用。Objective To study the relationship of urine RBC morphology between UF-100 urine sediment analytic instrument and phase contrast microscope. Methods The UF-100 urine sediment analytic instrument to analyze 500 urine specimens and study the relationship of urine RBC morphology between urine sediment analytic instrument and phase contrast microscope. Results The according perception of Normocytic, Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC- info are 91.4%, 94.4%, 83.3% respectively, the according perception between phase contrast microscope and RBC-P70FSC are 94. 9%, 95.7%, 94.7% respectively, and the according perception between phase contrast microscope and RBC FSC-DW are 84.4%, 86. 8%, 90. 5% respectively, the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are 84. 3%, 88. 1% and 83.3%, 87. 9% respectively. Conclusion The results show that the UF-100 urine sediment analytic instrument is simply operating, fast and high accurate, and which can instruct clinical dignose, therapy and prognosis judgement.

关 键 词：流式细胞术 显微镜检查 相差 尿分析 红细胞 参考值 

分 类 号：R446.1[医药卫生—诊断学]