甲氨喋呤与养气血祛风湿方对II型胶原诱导性关节炎小鼠的抗炎作用研究  

Comparison of anti-inflammatory effects between methotrexate and Yangqixue Qufengshi Recipe on mice arthritis induced by collagen II

作　　者：李芬[1] 吴轰[1] 范松青[2] 邓军卫[3] 田静[1] 

机构地区：[1]中南大学湘雅二医院风湿免疫科,湖南长沙410011 [2]中南大学湘雅二医院病理科,湖南长沙410011 [3]中南大学湘雅二医院医学实验中心,湖南长沙410011

出　　处：《中国医师杂志》2006年第9期1199-1201,共3页Journal of Chinese Physician

摘　　要：目的探讨甲氨喋呤与养气血祛风湿方对II型胶原诱导性关节炎小鼠的抗炎作用差别。方法采用C57BL/6×DBA/1小鼠,复制II型胶原诱导性关节炎,通过分别给予甲氨喋呤或养气血祛风湿方干预,观察II型胶原诱导性关节炎的出现时间、II型胶原抗体的浓度以及病理评分的改变。结果甲氨喋呤与养气血祛风湿方对II型胶原诱导性关节炎的出现时间和II型胶原抗体的浓度无影响。但是甲氨喋呤可以减少软骨破坏[(1.60±1.51)分vs(3.56±1.33)分,P<0.05]和骨质侵蚀[(0.30±0.69)分vs(1.67±1.50)分,P<0.01)];而养气血祛风湿方则减少骨质侵蚀[(0.22±0.44)分vs(1.67±1.50)分,P<0.01]。结论甲氨喋呤减少软骨破坏和骨质侵蚀,而养气血祛风湿方只减少骨质侵蚀,说明甲氨喋呤与养气血祛风湿方抗炎作用靶点有差异。Objective To compare the anti-inflammatory effects between methotrexate （MTX） and Yangqixue Qufengshi Recipe （YQXQFSR） on mice arthritis induced by collagen Ⅱ. Methods The model of rheumatoid arthritis was induced by using collagen Ⅱ in C57 BL/6 × DBA/1 mice, The rats with arthritis were treated with MTX or YQXQFSR respectively, The indexes such as the onset day of collagen induced arthritis （CIA） , the level of CⅡ-reactive antibodies and the pathological scores of CIA were assessed, Results The onset day of CIA and the level of CⅡ-reactive antibodies were not influenced in the treatment of mice arthritis with MTX and YQXQFSR respectively. But MTX could alleviate the damage of cartilage [ （ 1.60 ± 1.51 ） vs （ 3.56 ± 1.33 ） scores, P 〈 0. 05 ] and bone erosion [ （ 0. 30 ± 0. 69 ） vs （ 1.67 ± 1.50） scores, P 〈 0. 01 ]. YQXQFSR could only alleviate bone erosion [ （0. 22 ± 0. 44 ） vs （ 1.67 ± 1.50） scores, P 〈 0. 01 ]. Conclusion MTX can alleviate the damage of cartilage and bone erosion, and YQXQFSR can alleviate bone erosion. The mechanisms of MTX and YQXQFSR on the treatment of rheumatoid arthritis are different.

关 键 词：甲氨蝶呤 中药疗法 关节炎 实验性 

分 类 号：R285[医药卫生—中药学] R96[医药卫生—中医学]