西藏珞巴族15个STR位点遗传多态性研究  

Study of polymorphism on 15 STR loci of Luoba ethnic group from Tibet

作　　者：袁东亚[1] 康龙丽[1] 赵健民[2] 李生斌[3] 

机构地区：[1]西藏民族学院医学系,陕西咸阳712082 [2]西藏大学医学院,西藏拉萨850000 [3]西安交通大学医学院卫生部法医学重点实验室环境与疾病相关基因教育部重点实验室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2006年第4期319-323,336,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：教育部科技基础条件平台项目(No.505015);西藏自治区科技厅重大项目(No.05-53)

摘　　要：目的选择具有高度遗传多态性与稳定性的15个STR位点,进行西藏珞巴族、拉萨藏族、昌都藏族与亚洲其他人群的遗传关系分析。方法收集西藏珞巴族、拉萨和昌都藏族无关个体血样,利用AmpF/STR Identifiler试剂对样本DNA进行多重PCR扩增,产物在ABI 3100遗传分析仪上进行毛细管电泳和基因扫描及分型,并结合文献资料与中国其他21个民族群体、亚洲6个人群进行比较,绘制遗传树,分析西藏各民族与其他亚洲人群间的遗传关系。结果八个汉族群体首先聚类,广西五个民族首先聚类,两者共同与西藏珞巴族、拉萨藏族和昌都藏族聚类后,再与中华其他民族聚类,最后与亚洲6个人群及中国维吾尔族聚类。结论研究结果与各人群地理分布和历史基本一致,为研究珞巴族和藏族的起源、迁移、形成和发展提供遗传学依据。Objective To select 15 short tandem repeat （STR） loci that are highly and steadily polymorphic, and study the genetic relationships between Luoba ethnic group, Tibetan living in Lassa and Changdu area, and other Asian populations by using 15 STR polymorphism. Methods The blood samples of unrelated individuals from Luoba ethnic group, Lassa Tibetan and Changdu Tibetan were collected. DNA templates were screened using AmpF/ STR Identifiler kit and ABI3100Avant DNA analyzer with Genescan and Genotype software. The STR data of Luoba, Lassa Tibetan, Changdu Tibetan, other 21 Chinese ethnic groups and six Asian populations were compared; phylogenesis tree was constructed; the genetic relationships among ethnic groups living in Tibet and other Asian populations were analysed. Results Eight Hans clustered, and five ethnic groups of Guangxi clustered firstly; then the two clustered with Luoba, and two groups of Tibetan secondly; and then clustered with other Chinese populations, and then the Chinese groups clustered with other six Asian populations and Chinese Uygur lastly. Conclusion The results of this study are basically consistent with geographic distribution, and histories of these ethnic groups. They can provide the genetic proofs for the origin, migration, formation and development of ethnic groups living in Tibet.

关 键 词：珞巴族 短串联重复序列(STR) 遗传多态性 民族起源 进化树 

分 类 号：Q347[生物学—遗传学]