全反式维甲酸对肾间质纤维化大鼠肾间质巨噬细胞浸润的影响  

Effect of all-trans-retinoic acid on tubulointerstitial macrophage infiltration in rats with renal tubulointerstitial fibrosis

作　　者：朱丹[1] 高登峰[2] 宁宁[1] 桂保松[1] 姚钢炼[1] 卫小红[1] 周琳[1] 安雪芬[1] 

机构地区：[1]西安交通大学医学院第二附属医院肾内科,陕西西安710004 [2]西安交通大学医学院第二附属医院心内科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2006年第4期365-367,371,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：西安市科技局社会发展项目(No.GG04141)

摘　　要：目的探讨全反式维甲酸(all-trans-retinoic acid,at-RA)对肾间质纤维化大鼠肾间质巨噬细胞浸润的影响。方法采用单侧输尿管结扎(unilateral ureteral obstructive,UUO)大鼠模型。40只大鼠随机分为假手术组、模型组、苯那普利组、维甲酸小剂量组、维甲酸大剂量组。于造模前1 d至造模后14 d分别给予维甲酸小剂量组、维甲酸大剂量组、苯那普利组全反式维甲酸10 mg/(kg.d)2、0 mg/(kg.d)和苯那普利10 mg/(kg.d)。假手术组、模型组给予等量的生理盐水。用免疫组化染色观察肾间质巨噬细胞数和Ⅲ型胶原(collagenⅢ,ColⅢ)的表达。并常规行HE和Masson染色观察肾小管间质损伤指数。结果维甲酸和苯那普利均能显著抑制肾间质巨噬细胞的浸润(P<0.01),并且减低肾小管间质损伤指数和ColⅢ的表达(P<0.05)。结论维甲酸及苯那普利可能通过抑制肾间质巨噬细胞浸润而减轻UUO大鼠的肾损害。Objective To investigate the effect of at-RA in macrophage accumulation in tubulointerstitium of rats with renal tubulointerstitial fibrosis. Methods Unilateral ureteral obstructive（UUO） rat animal models were used for the study. 40 SD rats were randomly divided into 5 groups： sham group, UUO group, benazepril group, low-dose at-RA groups and high-dose at-RA groups. The rats were under intragastric administration by benazepril 10 mg/（kg·d） in benazepril group, and by at-RA 10 mg/（kg·d） in low-dose at-RA group and 20 mg/（kg·d） in high-dose at-RA group and by sodium chloride in tales doses in sham group and UUO group from the day before the operation to 14 day after operation. Immunohistochemistry staining of CD68 and Col Ⅲ was used to define the macrophage accumulation and expression of interstitial Col Ⅲ. The degree of tubulointerstitial damage was scored by HE and Masson staining. Results Tubulointerstitial macrophage infiltra＇tion were all significantly reduced by at-RA or benazepril treatment. They also improved the histological changes of UUO rats and inhibited interstitial colⅢ deposition. Conclusion Reduction of interstitial macrophage infiltration may be an important event by which at-RA or benazepril prevents renal injury caused by UUO.

关 键 词：肾间质 纤维化 巨噬细胞 胶原Ⅲ 全反式维甲酸 

分 类 号：R587.2[医药卫生—内分泌]