曲匹地尔对兔动脉球囊损伤后内膜增生和载脂蛋白J表达的影响  

Effect of trapidil on intima proliferation and the expression of apolipoprotein J in rabbit aortic artery after balloon injury

作　　者：陈海峰[1] 浦晓东[1] 谢良地[1] 高凌云[1] 郑伟[1] 林亚洲[1] 林立芳[1] 胡锡衷[1] 

机构地区：[1]福建医科大学省立临床医学院心内科,福州市350001

出　　处：《中华老年医学杂志》2006年第8期614-617,共4页Chinese Journal of Geriatrics

摘　　要：目的观察曲匹地尔对球囊损伤后血管内膜增生和载脂蛋白J(apoJ)表达的影响,探讨其抑制内膜增生的可能机制。方法纯种雄性日本大耳白兔90只,随机分成3组(每组30只):假手术组、球囊损伤组和曲匹地尔组,假手术组和球囊损伤组从术前7 d开始给予肌肉注射生理盐水1 ml·kg-1·d-1,曲匹地尔组肌肉注射5％曲匹地尔1 ml·kg-1·d-1。在光镜下观察曲匹地尔对兔腹主动脉球囊损伤的模型血管内膜增生的影响,应用逆转录多聚酶链反应和免疫组化的方法观察球囊损伤后apoJ的动态表达及曲匹地尔对其表达的影响。结果球囊损伤后第1、4、7、14、28天apoJ mRNA和蛋白表达都显著高于假手术组,且分别在损伤后第1天(3．82±0．36和0．64±0．16, P<0．01),和第14天(46．21±10．37和5．50±1．65,P<0．01)表达最为明显。曲匹地尔可以抑制血管内膜的增生,并能抑制apoJ mRNA和蛋白的表达。结论球囊损伤后apoJ的表达可能与血管内膜增生有关。曲匹地尔抑制血管内膜增生的作用可能与其抑制apoJ的表达有关。Objective To evaluate the effect of trapidil on intima proliferation and the expression of apolipoprotein J （apo J） after balloon injury. Methods On the model of balloon injury of rabbit aortic artery, the effect of trapidil on intima proliferation was observed under optical microscopy. The dynamic changes of apo J and the effect of trapidil on its expression were examined with the method of RT-PCR and immunohistochemistry. Results Compared with the control group, the transcription of apo J mRNA and expression of apo J protein were significantly increased at each time point after balloon injury, and the mRNA transcription level reached the maximum at the first day （3.82±0. 36 vs 0.64±0.16,P〈0.01）, protein expression reached the maximum at the fourteenth day（46.21 ± 10.37 vs 5.50 ±1.65, P 〈0.01） . Trapidil inhibited intima proliferation and the expressions of apo J mRNA and its protein. Conclusions Expression of apo J after balloon injury maybe correlates with intima proliferation. The mechanism by which trapidil suppresses the intima proliferation may be involved in the inhibitory effect on the expression of apo J.

关 键 词：曲匹地尔 载脂蛋白类 血管内膜 

分 类 号：R96[医药卫生—药理学]