低密度基因芯片技术检测人乳头瘤病毒基因型  

Detecting and Genotyping of Human Papillomavirus by Low-Density DNA Array

作　　者：王敏[1] 王方金[2] 吴健 王伟毅[2] 何蕴韶[1] 

机构地区：[1]中山大学中山医学院解剖学教研室,广东广州510080 [2]中山大学达安基因股份有限公司,广东广州510080 [3]广东省出入境检验检疫局,广东广州510623

出　　处：《中山大学学报（医学科学版）》2006年第4期431-434,共4页Journal of Sun Yat-Sen University:Medical Sciences

摘　　要：【目的】利用低密度基因芯片方法对人乳头瘤病毒(HPV)进行分型检测,建立一种经济实用的临床HPV感染的基因型检测方法。【方法】利用低密度基因芯片方法对145例阴道镜门诊病人的宫颈拭子标本进行HPV-DNA检测并分型。【结果】被检人群中共检测出57例HPV阳性,88例HPV阴性,HPV的感染率为39.3%,阳性标本中检测出12种高危型别,2种低危型别。其中单型感染51例(89%),混合感染6例(11%),16,31,18,58型检出率较高。【结论】低密度基因芯片是一种快速、简便、特异性强、灵敏度高的检测方法,在HPV和宫颈癌的筛查与诊断中有很大的应用价值。[Objective] To establish a high efficient and simple method for detecting and genotyping HPV from clinical sample. [Methods] Cervical scrape samples were collected from 145 patients who attending the clinic of colposcopy. DNA was extracted and amplified by PCR with general primer set. All PCR-positive samples were then genotyped by low-density DNA array. [Results] HPV-DNA was detected in 39.3% of the samples by low-density DNA array. Fifty-one patients were infected with single-type HPV and 6 patients were infected with multiple-type HPV. Twelve high risk types （16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66） and 2 low risk types （6, 11） were detected. The most commonly found high-risk types were HPV-16, HPV-31, HPV-18, and HPV-58. [Conclusion] Low-density DNA array is a rapid, convenient method for detecting of HPV DNA with high sensitivity and high specificity in diverse clinical sample. It is a valuable method for the molecular diagnosis and epidemiology of this important virus.

关 键 词：人乳头瘤病毒 低密度基因芯片 基因分型 

分 类 号：R373.1[医药卫生—病原生物学]