慢性乙型肝炎和乙型肝炎病毒携带儿童差异表达基因分析  

Analysis of Differentially Expressed Genes between Chronic Hepatitis B and Asymptomatic Hepatitis B Virus Carrying in Children

作　　者：唐翠兰[1] 周舟[2] 陈智[1] 路成吉[2] 

机构地区：[1]浙江大学医学院第一附属医院传染病研究所,杭州310031 [2]新乡医学院第三附属医院,河南新乡453003

出　　处：《实用儿科临床杂志》2006年第16期1088-1089,共2页Journal of Applied Clinical Pediatrics

基　　金：浙江省科技厅重大项目资助(2003C13015)

摘　　要：目的探讨儿童慢性乙型肝炎(CHB)和儿童乙型肝炎病毒(HBV)携带者(ASCs)的致病机制。方法采用抑制消减杂交(SSH)技术构建两者外周血单个核细胞差异表达基因cDNA文库,得到差异表达基因β2微球蛋白(β2-MG)。采集34例CHB和41例ASCs儿童外周血血浆,用酶联免疫试验检测β2-MG水平。结果成功构建了儿童CHB和ASCs的差异表达文库,发现在CHB儿童中β2-MG和铁转运蛋白等炎症相关基因表达增高。证实了β2-MG在蛋白水平也存在相应的差异表达。结论β2-MG在CHB患儿中的高表达参与儿童CHB的发病。Objective To explore the difference of pathogenesis between chronic hepatitis B （CHB） and asymptomatic hepatitis B virus（HBV） carriers（ASCs） in children. Methods Subtracted libraries was constructed by suppression subtractive hybridization （SSH） from peripheral blood mononuelear cell （PBMC） of two groups, beta- 2 - mieroglobulin （β2 - MG） was screened as one of differentially expressed genes. Serum was collected from children with CHB and ASCs, the differential expression of β2 - MG was confirmed by enzyme imm^y, Results Subtractive libraries were constructed successfully, the differentially expression of β2 - MG and lactroferrin were up - regulated in CHB children. And the differentially expression of β2 - MG on the level of protein was confirmed. Condusion The upregulation of β2 - MG in CHB children is involved in the pathogenesis mechanisms.

关 键 词：肝炎 乙型 慢性 乙型肝炎病毒携带者 Β2微球蛋白 抑制消减杂交 

分 类 号：R725.1[医药卫生—儿科]