青心酮对RAW264.7细胞可溶性Toll样受体4表达及TNF-α的影响  

Effect of DHAP on the Expression of sTLR4 mRNA & Protein and Secretion of TNF-α in RAW264.7 Cell Line

作　　者：张代娟[1] 武少杰[2] 刘同美[1] 田华[1] 王一鹏[1] 段文卓[1] 叶笃筠[3] 

机构地区：[1]潍坊医学院病理生理学教研室,山东潍坊261042 [2]解放军八十九医院眼科 [3]华中科技大学同济医学院

出　　处：《潍坊医学院学报》2006年第4期264-265,i0002,共3页Acta Academiae Medicinae Weifang

基　　金：国家自然科学基金资助课题(课题编号:30200373)

摘　　要：目的探讨中药成分青心酮(3,4-dihydroxyacetophenone,DHAP)对RAW264.7细胞炎症反应时可溶性Toll样受体4(soluble Toll-like receptor 4,sTLR4)mRNA、蛋白表达及TNFα-分泌的影响。方法建立细胞炎症反应模型,用RT-PCR及Western blot分析sTLR4 mRNA及蛋白水平的改变,用ELISA方法检测TNFα-分泌的变化。结果RAW264.7细胞炎症反应时,sTLR4 mRNA及蛋白表达均下调,该细胞经青心酮处理后在炎症反应时,sTLR4 mRNA及蛋白表达均上调,与未处理组比较,表达明显增加,而TNFα-分泌下降。结论DHAP预处理能显著上调sTLR4 mRNA及蛋白表达,减少TNFα-分泌,抑制炎症反应。这将为临床治疗感染性休克提供一个新的研究方向。Objective To explore the effect of DHAP on the expression of soluble Toll-like receptor 4（sTLR4） mRNA, protein and secretion of TNF-α in RAW264.7 cell line. Methods Inflammation model was established. Changes of sTLR4 mRNA and protein were analyzed by RT-PCR and Western blot. Changes of TNF-α was detected by ELISA. Results During inflanunation, the level of sTLR4 mRNA and protein was decreased, after 4h stimulated hy LPS;the level of sTLR4 protein was increased, and secretion of TNF-α was decreased when the cell line was pretreated with DHAP. Conclusion The expression of sTLR4 could be significantly up-regulated in pretreated RAW264.7 cell line with DHAP, and TNF-α secretion could be decreased. These may provide a new therapy for inflammation shock in clinical practice.

关 键 词：青心酮 可溶性Toll样受体4 炎症反应 巨噬细胞 

分 类 号：R446.61[医药卫生—诊断学]