邻苯二甲酸二乙基己酯对新生小鼠睾丸及Leydig细胞影响的实验研究  

Di(2-ethylhexyl) Phthalate Affects the Testes and Leydig Cells of Neonatal KM Mice

作　　者：宋晓峰[1] 魏光辉[1] 邓永继[1] 陈璇[1] 刘星[1] 张德迎[1] 

机构地区：[1]重庆医科大学附属儿童医院泌尿外科,重庆400014

出　　处：《中华男科学杂志》2006年第9期775-779,共5页National Journal of Andrology

基　　金：国家自然科学基金项目(30371475)

摘　　要：目的：探讨邻苯二甲酸二乙基己酯（DEHP）对新生小鼠睾丸及Leydig细胞形态结构及功能的影响。方法：DEHP分别以低、中、高3组剂量[100、200、500mg／（kg·d）]灌胃作用于怀孕12d到产后3d（GD12～PND3）的KM母鼠，观察DEHP对新生雄性仔鼠体重、睾丸重量、Leydig细胞形态结构和3β-羟基类固醇脱氢酶（3β—HSD）活性、酶反应面积的影响。结果：DEHP作用于母鼠后，其雄性子代幼鼠体重和睾丸重量减轻，睾丸Leydig细胞形态、超微结构发生改变；高剂量组Leydig细胞数量明显增多；低、中剂量组睾酮合成关键酶3β-HSD酶活性下降，酶反应面积减小，但高剂量组在仔鼠出生后15d时酶活性降低[（吸光度值（0．154±0.011）1）8空白对照组（0．222±0.013），P〈0．01]，而酶反应面积增大[（6303．0±745．6）μm^2 vs 空白对照组（5091．4±214．4）μm^2，P〈0．01）]。结论：DEHP能影响新生雄性小鼠体重、睾丸重量、Leydig细胞的形态结构和3B—HSD活性，具有抗雄激素效应。Objective： To explore the effects of di（2-ethylhexyl）phthalate（DEHP） on neonatal mice＇s testes and Leydig cells in vivo. Methods： Pregnant mice were exposed to DEHP at the dose of 100 mg/kg, 200 mg/kg or 500 mg/kg（body weight） per day by gavage from gestation day 12（ GD 12） through postnatal day 3（ PND 3）, respectively. The testis and body weights, testicular histopathology and the activity of 3β-hydroxysteroid dehydrogenase （3β-HSD） of the neonatal mice were investigated. Results： The body and testis weights of the male mice＇s offspring were significantly reduced following DEHP exposure. Leydig cell morphology was affected significantly by DEHP as compared with the controls. Leydig cells obviously increased in the neonatal mice＇s testes on PND 15 and PND 30 when exposed to DEHP （500 mg/[ kg· d ] ）. Activities and positive area of the steroidogenic enzymes 3β-HSD immunoexpression decreased markedly when exposed to DEHP （ 100 mg/[ kg · d] or 200 mg/[ kg · d] ）. Image analysis showed a decrease in the activities of 3β-HSD in the animals exposed to DEHP （500 mg/[ kg · d ] ）, but an increase in the positive area of 3β-HSD immunoexpression as compared with the control animals on PND 15 （ P 〈 0.01 ）. Conclusion ： DEHP affects the Leydig cell morphology, the activity of 3β-HSD, the testis and body weights and the testicular histopathology of neonatal mice, and it may function as an antiandrogenic agent.

关 键 词：邻苯二甲酸二乙基己酯 胚胎 LEYDIG细胞 3β-羟基类固醇脱氢酶 小鼠 新生 

分 类 号：R114[医药卫生—卫生毒理学]