5氟脲嘧啶关节腔注射治疗兔膝骨性关节炎的实验研究  

Study of intra-articular injection with 5Fu in the treatment of rabbit knee osteoarthritis

作　　者：王巨[1] 肖德明[2] 李伟[2] 林博文[2] 徐忠世[2] 陈蓟[2] 吕猛[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022 [2]暨南大学第二临床学院骨关节科,深圳518020

出　　处：《中国矫形外科杂志》2006年第17期1339-1341,i0004,共4页Orthopedic Journal of China

摘　　要：[目的]评价5氟脲嘧啶(5-F luorourac il,5Fu)关节腔注射治疗兔膝骨性关节炎(osteoarthritis,OA)的疗效。[方法]24只兔子制成骨关节炎模型随机分成OA组、5Fu组和对照组,OA组立即处死,5Fu组按5Fu 2mg/kg关节腔注射,每周1次连续4次,对照组注射等量生理盐水,最后1次治疗后1周处死。观察3组滑膜组织的光镜、电镜改变及软骨的光镜、MMP-1免疫组化改变,比较软骨M ank in s评分及关节液中IL-1的浓度。[结果]5Fu组可见软骨破坏减轻,滑膜炎症明显抑制,M ank in s评分明显改善(P<0.01);关节液IL-1浓度降低(P<0.05),关节软骨中MMP-1表达减弱。[结论]5Fu关节腔内注射能抑制滑膜炎症,缓解软骨的破坏。[ Objective] To evaluate the effects of intra-artieular 5Fu injection in the treatment of rabbit knee osteoarthritis （OA） . [ Method] Twenty-four rabbits were randomly divided into 5Fu group, OA group and Control group after the model of OA established. Then OA group were sacrificed. 5Fu group and Control group were received intra-artieular 5Fu and physiologic saline injection respectively once a week for 4 weeks and sacrificed one week after last injection. Microscopic examinations were conducted to observe the pathological changes of articular cartilage and synovia and Mankin＇s scale were performed. The immunohistoehnieal analyses of MMP-ldistribution in cartilage were performed and the level of IL-1 of synovial fluid were determined by ELISE. [ Result] The destruction of cartilage was alleviated and synovialitis was depressed in 5Fu group. The Mankin＇s scale of the group was significantly decreased （P 〈0. 01 ）. The level of IL-1 of synovial fluid decreased （P 〈0. 05 ） and the expression of MMP-1 in the cartilage of 5Fu group decreased as well. [ Conclusion] 5Fu can control the inflammation of synovia and alleviate the destruction of cartilage.

关 键 词：骨性关节炎 滑膜炎 5氟脲嘧啶 白介素-1 金属蛋白酶-1 

分 类 号：R684.3[医药卫生—骨科学]