TNF-α对人脐静脉内皮细胞中NO和eNOS的影响  

Effect of TNF-α on production of NO and Activity of eNOS in human umbilical vein endothelial cells

作　　者：吴军[1] 李闪[1] 朱建华[1] 傅婷[1] 

机构地区：[1]浙江大学医学院附属第一医院心内科

出　　处：《浙江医学》2006年第8期636-638,684,共4页Zhejiang Medical Journal

摘　　要：目的讨论肿瘤坏死因子-α(TNF-α)对内皮细胞一氧化氮(NO)产生及内皮型一氧化氮合酶(eNOS)活性的影响。方法以人脐静脉内皮细胞(HUVEC)为实验材料,检测与不同浓度TNF-α作用不同时间后,细胞培养上清液和细胞中NO水平的变化,以及细胞eNOS活性的改变。结果(1)随着TNF-α浓度的升高,eNOS的活性减弱,而细胞和上清液中NO的含量增加;(2)随着干预时间的延长,eNOS的活性减弱;而细胞和上清液中NO的含量在作用24h后升高,且明显高于对照组;(3)L-单甲基精氨酸(L-NMMA)和地塞米松(DXM)均能阻断TNF-α引起的细胞和细胞上清中NO的表达增加。结论TNF-α降低eNOS活性,却在高浓度和长时间作用后增加NO的合成,可能与激活诱导型一氧化氮合酶有关,因为NO的变化可以被L-NMMA和DXM阻断。Objective To investigate the effect of TNF-α on production of nitric oxide （NO） and activity of nitric oxide synthase（eNOS） in human umbilical vein endothelial cells（HUVECs）. Methods HUVECs were incubated with TNF-α at different concentrations （2-20ng/ml） and different times（2-48h） or pretreatment with L-NMMA or DXM. Nitric oxide production and activity of nitric oxide synthase in HUVECs were measured by NO and NOS assay kits. Resuits （1） Activity of eNOS was down-regulated by TNF-α in a concentration-dependent manner, while production of NO was upregulated. （2） The activity of eNOS attenuated after long-time treatment with TNF-α, but marked increased production of NO was detected after 24h. （3） The induction of NO by TNF-α can be inhibited by L-NMMA and DXM. Conclusion TNF-α can reduce activity of eNOS and induce iNOS, so that production of NO is increased.

关 键 词：肿瘤坏死因子-Α 人脐静脉内皮细胞 一氧化氮 内皮型一氧化氮合酶 

分 类 号：R543[医药卫生—心血管疾病]