刺参岩藻聚糖含量的研究  

Study on fucoidan of Stichopus japonicus

作　　者：李兆新[1] 刘晋湘[2] 耿霞[1] 王慧[2] 刘莹[2] 李兰生[2] 刘彬[1] 张剑诚 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]中国海洋大学,青岛266003 [3]大连太平洋海珍品有限公司,116045

出　　处：《海洋水产研究》2006年第4期44-47,共4页Marine Fisheries Research

基　　金：国家科技攻关计划项目(2002BA906A39)资助

摘　　要：以木瓜蛋白酶水解比色法测定刺参中岩藻聚糖的含量。水解液经次甲基蓝染色,在560nm处有最大吸收波长。吸光度与刺参中岩藻聚糖含量成正比。测定的刺参酸性粘多糖回归方程:y=-0.0189+0.0345x;r=0.9991。并研究了6种不同产地的刺参中岩藻聚糖的含量。Hydrolization method with papain and colorimetric analysis were used for determining the content of fucoidan in Stichopus japonicus. On the basis of absorbance and concentration, the regression equation was acquired, viz. y=-0. 018 9＋0. 034 5 x, r=0. 999 1. In addition, acid mucopolysacharide in 6 Stichopus from different areas was discussed.

关 键 词：刺参 岩藻聚糖 次甲基蓝 含量 

分 类 号：Q538[生物学—生物化学]