发育期大鼠高热惊厥脑损伤CO变化及Znpp对其影响  

The carbon monoxide change in febrile convulsion brain damage in developing rats and effects of Znpp on it

作　　者：王显鹤[1] 顾镜月[1] 吴祥红[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154002

出　　处：《黑龙江医药科学》2006年第4期53-54,共2页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省教育厅科学技术研究项目(11511400)

摘　　要：目的:研究锌原卟啉对大鼠高热惊厥脑损伤CO变化的影响及作用机制。方法:21日龄W istar大鼠,随机分为对照组、高热未惊厥组、FC组及Znpp治疗组。采用热水浴诱导FC大鼠动物模型和Znpp治疗模型。用分光光度计检测各组大鼠脑组织匀浆和血浆CO含量,用HE染色观察大鼠海马神经元形态学改变。结果:FC组血浆CO含量明显升高,与对照组、高热未惊厥组及Znpp治疗组比较,有显著性差异(P<0.01),Znpp治疗组血浆CO含量升高与高热未惊厥组比较,亦有显著性差异(P<0.05);FC组脑组织匀浆CO含量增高,与对照组、高热未惊厥组及Znpp治疗组比较有显著性差异(P<0.01),Znpp治疗组脑组织匀浆CO含量升高,与对照组、高热未惊厥组比较有显著性差异(P<0.01),高热未惊厥组与对照组比较差异无显著性(P>0.05);结论:FC能够引起脑损伤且CO水平增高,Znpp能够使CO水平下降,对脑损伤有保护作用。Objective：The main purpose of the present study was to explore the effect of Znpp on the carbon monoxide（CO） change and the brain damage caused by febrile convulsion （ FC ）in rat. Methods： FC in rats was induced by hot water bath, ten times, once every 2 days. 21- day Wistar rats were randomly divided into two groups ,The latter was further divided into none FC group （n= 16） ,FC group（n =20）and Znpp treatment groups（n=15）. HE-staining was used to detect the morphologic changes of hippocampal neurons. The indirect CO production in the brain and plasma was detected by the spectrophotometer. Results： The brain tissue homogenate and plasma CO concentration were significantly increased in FC group as compared with that in normal group,none FC group and Znpp treatment group （P 〈 0.01） . The expression of HO-1 mRNA was significantly increased in the hippocampal CA1,CA3 region and dentate gyrus in FC group as compared with that in normal group, none FC group and Znpp treatment group. Conclusions：Recurrent FC in rats may cause brain damage and FC in rats can cause the CO increase. Znpp inhibits the CO to have a protective effect on FC brain damage.

关 键 词：锌原卟啉 高热惊厥 一氧化碳 脑 

分 类 号：R651[医药卫生—外科学]