血管内皮细胞生长因子调动内皮祖细胞修复动脉损伤的实验研究  

Experimental study of mobilizing endothelial progenitor cells to repair artery injury

作　　者：徐明国[1] 陶军[1] 王洁梅[1] 杨震[1] 陈龙[1] 涂昌[1] 王妍[1] 

机构地区：[1]中山大学附属第一医院,广东广州510080

出　　处：《中华老年心脑血管病杂志》2006年第9期624-627,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金资助项目(30470475)

摘　　要：目的探讨血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)调动内皮祖细胞(endothelial progeni-tor cells,EPCs)修复大鼠颈总动脉损伤的作用。方法将32只大鼠切除脾脏恢复两周后随机分为两组:VEGF组和对照组,每组16只。用2F的Fogarty球囊导管损伤大鼠左颈总动脉建立动脉损伤模型。用流式细胞仪测定大鼠外周血胎儿肝脏激酶-1+(fetal liver kinase,Flk-1+)的细胞比例。动脉损伤两周后取损伤动脉段和对侧正常动脉段,采用组织学、免疫组织化学、扫描电子显微镜和图像分析技术评价各组动脉修复的效果。结果VEGF组Flk-1+的细胞比例显著高于对照组[(38.59±3.68)%vs(13.78±1.91)%,P<0.05]。两组中相同数目的外周血单个核细胞分化为EPCs的数目,VEGF组显著高于对照组[(28.25±4.23)个vs(6.88±1.34)个,P<0.05]。动脉损伤两周后,VEGF组损伤动脉的新生内膜厚度显著低于对照组[(32.38±5.58)μmvs(76.38±5.48)μm,P<0.05]。结论VEGF具有调动EPCs修复大鼠颈总动脉损伤的作用。Objective To investigate whether vascular endothelial growth factor（VEGF） can mobilize endothelial progenitor cells（EPCs） to repair the rat common carotid artery injury. Methods Thirty-two splenectomized rats were divided into two groups randomly： VEGF group and control group of 16 rats each. The rat left common carotid artery injury model was established using 2F Fogarty catheter. The rat peripheral blood was col- lected to detennine the proportion of Flk-1^＋ positive cells in peripheral mononuclear cells with flow cytometry. After two weeks the repair of injured artery was analyzed with pathology, immunohistochemistry, scanning electron microscope and morphometry. Results The proportion of Flk-1^＋ positive cells in VEGF group was signif- icantly higher than that in control group [ （38.59 ± 3.68） % vs （ 13.78± 1.91） %, P 〈 0.05t. There were significantly more EPCs differentiated from equal peripheral mononuclear cells in VEGF group than that in control group [ （28.25 ± 4.23） cells vs （6.88 ± 1.34） cells, P 〈 0.05 ]. The neointimal thickness in VEGF group was significantly lower than that in control group [（32.38 ± 5.58） tan vs （76.38±5.48） tan, P 〈 0.05 ]. Conclusion VEGF can mobilize EPCs to repair the rat carotid arterial injury.

关 键 词：内皮 血管 内皮生长因子 颈动脉损伤 大鼠 

分 类 号：R543.5[医药卫生—心血管疾病]