血液透析过程中患者白细胞介素-8血浆水平及基因表达的改变  

Changes of plasma interleukin-8 level and gene expression of the uremic patients during the hemodialysis

作　　者：吕路[1] 黄锋先[2] 管红斌[1] 张增华[1] 桓文穆[1] 黄华[1] 钟亮尹[1] 蔡小燕[1] 

机构地区：[1]广东药学院附属第一医院肾内科,广州510080 [2]中山大学附属第一医院肾内科

出　　处：《中国医药》2006年第9期544-546,共3页China Medicine

基　　金：广东省医学科学技术研究基金(A2005513)

摘　　要：目的探讨血液透析过程中尿毒症患者白细胞介素8(IL8)血浆水平及基因表达的改变。方法用酶联免疫吸附法测IL8血浆水平,逆转录多聚酶链反应测基因表达。结果患者透析后的血浆IL8水平及外周血单个核细胞(PBMC)的IL8mRNA表达均较血液透析前升高,透析15min时用再生纤维素膜的患者PBMC的IL8mRNA表达较血液透析前减少。铜仿膜导致IL8基因表达改变的能力强于聚砜膜。结论血液透析可引起尿毒症患者IL8血浆水平及PBMC的IL8mRNA表达的异常,测量IL8血浆水平及基因表达有助于反映不同透析膜的生物相容性。Objective To investigate the changes of the plasma interleukin-8 level（ IL-8 ） and gene expression of the uremic patients during the hemodialysis. Methods The plasma concentration of IL-8 was measured with enzyme-linked immunoabsorbent assay （ ELISA ）. The gene expression of IL-8 was measured with reverse transcription-polymerase chain reaction （RT-PCR）. Results The IL-8 plasma level and IL-8 mRNA expression of the peripheral blood mononuclear cells （ PBMC ） increased after hemodialysis. Through the dialysis course, the IL-8 mRNA expression of PBMC at 15 min was lower than that at the beginning in the regenerated cellulose membrane groups. The change of IL-8 mRNA expression of PBMC through the hemodialysis in cuprophan membrane group was more significant than that in polysulfone membrane group. Conclusion Hemodialysis can lead to the abnormal plasma IL-8 level and IL-8 mRNA expression of PBMC in the uremic patients. The measurement of plasma IL-8 level and IL- 8 mRNA expression of PBMC is useful to evaluate the biocompatibility of dialysis membranes.

关 键 词：尿毒症 血液透析 白细胞介素-8 透析膜 

分 类 号：R692[医药卫生—泌尿科学]