以纳米金为介质的无标记电流型甲胎蛋白免疫传感器的研究  

Gold Nanoparticles Modified Biosensor for Detection of α-Fetoprotein(AFP)

作　　者：朱强[1] 袁若[1] 柴雅琴[1] 黎雪莲[1] 王娜[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2006年第1期81-85,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(29705001);教育部优秀青年教师资助计划项目(200240);重庆市自然科学基金资助项目(20027477)

摘　　要：利用电沉积方法将纳米级的金溶胶沉积在玻碳（GC）电极表面．再自组装固定甲胎蛋白抗体（anti-AFP），研制成AFP免疫传感器．研究了电极修饰过程中的纳米金沉积时间、抗体吸附时间、测试液pH值、温度等实验条件和参数对传感器性能的影响．该传感器在AFP浓度为2～60ng／mL的范围内有良好的线性关系并且制作简单，响应快，能满足实际测定的要求．Gold nanoparticles were electrodeposited on the surface of glass carbon （GC） electrode, then an ti-AFP was absorbed by gold nanoparticles to prepare a biosensor. The effect of electrodeposition time and absorption time on the electrochemical behavior of biosensor was studied. Other experimental conditions such as pH and temperature were studied too. The current response of the biosensor is in linear relation ship with concentration of AFP in the range of 2 ng/mL-60 ng/mL. This approach has some advantage such as simple, a short response time and a satisfied detection range.

关 键 词：甲胎蛋白 金溶胶 免疫传感器 玻碳电极 

分 类 号：O657.1[理学—分析化学]