环境镉暴露时胎盘组织学改变与MT表达的研究  

Influence of Environmental Exposure to Cadmium on Placental Tissue Structure and Metallothionein Expression.

作　　者：赵永成[1] 孙勇[2] 于民[3] 齐雪岭[3] 王继先[1] 周晓芹[3] 郭国庆[3] 吴叶菊[3] 高春英[3] 王耐芬[4] 

机构地区：[1]中国医学科学院放射医学研究所,天津300192 [2]新疆医科大学公共卫生学院 [3]新疆克拉玛依市中心医院 [4]北京大学医学部公共卫生学院

出　　处：《中国辐射卫生》2006年第3期264-266,共3页Chinese Journal of Radiological Health

基　　金：国际原子能机构资助项目(编号11933/R0);高等学校博士学科点专项科研基金(编号:20020023039)

摘　　要：目的研究环境镉暴露时胎盘组织变化及MT在胎盘组织中表达的意义。方法于2002年11月～2003年4月调查湖北省某市的待产孕妇情况，分娩时收集孕妇全血、脐血和胎盘标本用于测定镉含量。胎盘组织经常规病理学检查后用免疫组织化学方法检测胎盘组织MT表达情况，进一步分析环境镉暴露孕妇胎盘组织中MT与镉的关系。结果120例20～34岁孕妇血镉、胎盘镉和脐血镉波动范围分别为0．27～13．00μg/L、0．033～1．69μg/g（干重）和0．13～2．69μg／L，脐血镉水平均低于母血镉。统计分析显示MT不同表达组间胎盘组织病理学变化有显著差异。对数线性模型分析显示胎盘的组织学改变可能是MT与母血镉交互作用的结果。结论镉暴露对胎盘组织结构会产生影响，同时镉又能诱导胎盘组织MT表达，MT能使胎盘的适应性反应增强，进而减轻镉对胎盘组织的损伤。Objective To investigate the Significance of Metallothionein Expression in the Placental of Population Environmental Exposed to Cadmium. Methods Pregnant women were chosen from one city of Hubei province from November 2002 to April 2003. Whole blood of the pregnant women, umbilical cord blood of neonates and placenta were collected and cadmium levels were determined by inductively coupled plasma emission mass spectroscopy. Immanohistocbemistry staining and S - P method were used to observe the expression of the MT in placental tissue from 120 cases of human term placenta to analyze the potential association between MT and placental cadmium or umbilical cord blood cadmium in the pregnant women of environmental exposure to cadmium. Results Matemal blood cadmium in 120 healthy pregnant women flucatuced from 0.27 to 13.00 μg/L and placental flucatualed from 0.033 to 1.69 μg/g dry weight. Cord. blood cadmium flucatuced from 0.13 to 2.69 μg/L, all of which were lower than those significantly in matemal blood. Statistics analysis showed that the placental histopathologicd change in the different MT expression group differs significantly. Loglinear models analysis showed that the placental tissue change was probably associated with MT and maternal blood cadmium interaction. Conclusion Results show that, cadmium exposure influences the structural changes in the placenta, and induces the of MT expression in placental tissue. Placental adaptability reaction was strengthened by MT, thus mitigating the trauma of the placental tissue.

关 键 词：金属硫蛋白 镉 胎盘 免疫组化 环境暴露 

分 类 号：R595[医药卫生—内科学]