活脉饮对实验性兔动脉粥样硬化病变中胶原及基质金属蛋白酶1的影响  

Effects of Huomai Yin on Collagen and Matrixmetalloproteinase-1 of Atherosclerosis in Rabbits

作　　者：李晓东[1] 刘红霞[1] 毛颖[1] 杨智勇[1] 

机构地区：[1]中国医科大学附属第二医院心内科,辽宁省沈阳市110004

出　　处：《中国动脉硬化杂志》2006年第2期115-118,共4页Chinese Journal of Arteriosclerosis

基　　金：辽宁省自然科学基金(20042079)

摘　　要：目的研究活脉饮对动脉粥样硬化的治疗作用。方法建立兔动脉粥样硬化模型,观察活脉饮治疗后动脉粥样硬化斑块的范围、斑块内胶原含量和基质金属蛋白酶1表达的变化,并与单纯高脂组和辛伐他汀组比较。结果活脉饮高剂量组(每天12 g/kg)斑块范围较单纯高脂组明显减小(P<0.01),与辛伐他汀组比较差异无显著性(P>0.05),活脉饮低剂量组(每天6 g/kg)与单纯高脂组相比无明显差异(P>0.05)。胶原含量活脉饮高、低剂量组均较单纯高脂组减少(P<0.01,P<0.05),高剂量组与辛伐他汀组比较差异无显著性(P>0.05),低剂量组胶原含量较辛伐他汀组高(P<0.05)。基质金属蛋白酶1的表达活脉饮高剂量组与辛伐他汀组比较差异无显著性,较单纯高脂组明显增多(P<0.01),低剂量组与高脂组差异无显著性(P>0.05)。结论活脉饮呈剂量依赖性抗兔动脉粥样硬化,其机制之一可能是通过增强基质属蛋白酶的表达、减少斑块内胶原的聚集而发挥作用。Aim To study the effect of Huomai Yin on atherosclerosis. Methods The experimental model of atherosclerosis in rabbits was established by endothelial injury by balloon and hyper-lipid feed. The atheroslerosis plaque area, collagen and matrixmetalloproteinase-1 differences between groups were evaluated at the end of the study. Results At the end of 8 weeks after endothelial injury by balloon, the plaque area in Huomai Yin highdose group（every day 12 g/kg） was smaller than that in hyper lipid group（ P 〈 0.01 ） ; compared with simvastafin group, the plaque area in highdose group was larger, but the difference wasn＇t significant（ P〉 0.05）; compared with hyper lipid group, the plaque area in lowdose group（every day 6 g/kg） wasn＇ t significantly smaller（ P 〉 0.05 ）. Collagen was reduced in highdose group and lowdose group than in hyper lipid group（ P 〈0.01, P〈0.05）. Collagen wasn＇t significantly different between highdose group and simvastatin group （P〉0.05）, but collagen in lowdose group was more than simvastatin group （ P 〈 0. 05 ）. The express of matrixmetalloproteinase- 1 in highdose group and simvastatin group wasn＇t significantly different（ P 〉 0.05）, but was higher than that in hyper lipid group（ P 〈 0.01）. Condusions Huomai Yin has therapeutic effect on atheroselerosis in rabbit. Huomai Yin could modulate the express of 1 and decrease collagen accumulation in the atherosclerosis plaque

关 键 词：内科学 活脉饮抗动脉粥样硬化 免疫组织化学 细胞外基质 胶原 基质金属蛋白酶 兔 

分 类 号：R541.4[医药卫生—心血管疾病]