胰岛素样生长因子1受体抗体对大鼠血管平滑肌细胞增殖的影响  

Effect of Insulin Like Growth Factor-1 Receptor Antibody on Rat Carotid Smooth Muscle Cell Proliferation

作　　者：苏海霞[1] 盛净[1] 刘德莉[1] 夏万尧[1] 成静[1] 陈朝婷[1] 

机构地区：[1]上海第二医科大学附属第九人民医院老年科,上海市200011

出　　处：《中国动脉硬化杂志》2006年第2期137-139,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的观察胰岛素样生长因子1受体抗体对大鼠血管平滑肌细胞增殖的影响。方法球囊导管损伤大鼠一侧颈总动脉,7天后体外培养受损血管的中膜平滑肌细胞,以损伤侧血管细胞为实验组,以对侧正常血管中膜平滑肌细胞为对照组;采用-αactin免疫细胞化学法进行细胞鉴定;在细胞的培养悬液中加入不同浓度的胰岛素样生长因子1受体抗体作用24 h,然后采用5-溴-2脱氧尿苷细胞增殖检测法检测细胞的增殖程度。结果大鼠颈总动脉球囊损伤后,体外培养的中膜平滑肌细胞表现出显著的增殖性(与对照组比较,P<0.01);胰岛素样生长因子1受体抗体可以显著抑制大鼠平滑肌细胞的增殖,呈现出浓度依赖性特征(组间比较,P<0.01)。结论胰岛素样生长因子1受体抗体具有抑制平滑肌细胞增殖的作用,有望用于治疗平滑肌细胞增殖性疾病。Aim To study the effect of insulin-like growth factor- 1 receptor antibody on vascular smooth muscle cell proliferation. Methods Seven days after balloon injury, rat＇s carotid arteries were harvested, smooth muscle cell were cultured in vitro and were evaluated by a-actin immunocytochemical method. The effects of insulin-like growth factor-1 receptor antibody on smooth muscle cell proliferation were observed by BrdU immunocytochemical methods. Results Smooth muscle cell derived from injured arteries shows higher proliferefion compared with those from normal arteries（ P 〈 0.01 ）, insulin-like growth factor-1 receptor antibody monoclonal antibody can inhibit smooth muscle cell proliferation concentration dependently（ P 〈 0.01）. Conclusion Insulin-like growth factor-1 receptor antibody can inhibit smooth muscle cell proliferation, which deserves to be researched deeply.

关 键 词：内科学 抑制平滑肌细胞增殖 免疫细胞化学 胰岛素样生长因子1受体抗体 大鼠颈总动脉 再狭窄 

分 类 号：R5[医药卫生—内科学]