细胞外信号调节激酶通路激活抑制δ氨基酮戊酸-光动力疗法对SW480细胞的细胞毒作用  

Activation of extracelluar signal-regulated kinase pathway inhibits cytotoxic effect of SW480 cell in response to 5-aminolevulinic acid-based photodynamic therapy

作　　者：郑江华[1] 时德[1] 赵渝[1] 陈祖林[2] 

机构地区：[1]重庆医科大学附属第一医院血管外科,重庆400016 [2]第三军医大学附属新桥医院普外科,重庆400037

出　　处：《重庆医科大学学报》2006年第4期482-485,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(No.30271481)

摘　　要：目的:探讨δ氨基酮戊酸-光动力疗法(ALA-PDT)诱导SW480细胞后的细胞外信号调节激酶(ERK)通路以及阻断此通路对细胞毒作用的影响。方法:将SW480细胞分为空白对照组、激光照射组、ALA组、ALA-PDT组(又分为30、60、90min组)及PD98059组,Western蛋白印迹检测各组细胞MEK和ERK1/2蛋白产物及磷酸化蛋白产物的表达;用MTT比色法分别检测各组细胞在不同时间段的光密度值,计算各组细胞存活率。结果:ALA-PDT后SW480细胞的ERK通路激活;阻断ERK通路后ALA-PDT对SW480细胞的细胞毒作用增强。结论:ERK通路的激活对ALA-PDT后SW480细胞起着保护作用;不同水平阻断ERK通路,可能成为增强ALA-PDT杀伤结肠癌细胞的新靶点。Objective： To investigate extracelluar signal-regulated kinase（ERK） pathway and cytotoxic effect after block of this pathway after 5-aminolevulinic acid（ALA） -based photodynamic therapy （PDT） on SW480 cell. Methods： SW480 was divided into control group, light group, ALA group, ALA-PDT group and PD98059 group. Western blot was used to detect the expression of protein products and phosphorylation protein products of MEK and ERK1/2. Optical density value and survival rate of each group were obtained at different time by MTF. Results： ERK pathway of SW480 cell was activated and block of this pathway increased cytotoxic effect of SW480 cell after ALA-PDT. Conclusions： Activation of ERK pathway protects SW480 cell from ALA-PDT. Block of ERK pathway at different levels may become a new target, which will enhance cytotoxic effect of ALA-PDT on colon carcinoma.

关 键 词：δ氨基酮戊酸 光动力疗法 SW480 细胞外信号调节激酶通路 

分 类 号：Q27[生物学—细胞生物学]