Alkaline Protease Production by a Strain of Marine Yeasts  

作　　者：WANG Ping CHI Zhenming MA Chunling 

机构地区：[1]UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao 266003, P. R. China

出　　处：《Journal of Ocean University of China》2006年第3期263-268,共6页中国海洋大学学报（英文版）

基　　金：The authors would like to thank the National Natural Science Foundation of China for providing the financial support for the study （No. 30328021）.

摘　　要：Yeast strain 10 with high yield of protease was isolated from sediments of saltern near Qingdao, China. The protease had the highest activity at pH 9.0 and 45 ℃. The optimal medium for the maximum alkaline protease production of strain 10 was 2.5 g soluble starch and 2.0 g NaNO3 in 100 mL seawater with initial pH 6.0. The optimal cultivation conditions for the maximum protease production were temperature 24.5 ℃, aeration rate 8.0 L min -1 and agitation speed 150 r min -1. Under the optimal conditions, 623.1 U mg -1 protein of alkaline protease was reached in the culture within 30 h of fermentation.Yeast strain 10 with high yield of protease was isolated from sediments of saltern near Qingdao, China. The protease had the highest activity at pH 9.0 and 45℃. The optimal medium for the maximum alkaline protease production of strain 10 was 2.5 g soluble starch and 2.0 g NaNO3 in 100 mL seawater with initial pH6.0. The optimal cultivation conditions for the maximum protease production were temperature 24.5 ℃, aeration rate 8.0 L min^- 1 and agitation speed 150 r min^-1 . Under the optimal conditions, 623.1 Umg^-1 protein of alkaline protease was reached in the culture within 30 h of fermentation.

关 键 词：marine yeasts alkaline protease FERMENTATION optimal conditions 

分 类 号：Q178.2[生物学—水生生物学]