表达传染性喉气管炎病毒gB基因和新城疫病毒F基因重组病毒免疫及强毒攻击后外周血T淋巴细胞亚群的动态  

Kinetics of lymophocyte subsets in peripheral blood of chickens inoculated with recombinant fowl pox virus expressing gB gene of larngotracheitis virus and fusion gene of newcastle disease virus and after challenge

作　　者：智海东[1] 王云峰[1] 孙永科[2] 张晶[1] 王玫[1] 彭金美[1] 刘永刚[1] 童光志[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]西北农林科技大学动物医学系,陕西杨凌712100

出　　处：《中国预防兽医学报》2006年第5期535-538,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家"863"计划资助(No.2003AA213020)

摘　　要：采用流式细胞检测技术对表达传染性喉气管炎病毒gB基因和新城疫病毒F基因重组鸡痘病毒(rFPV-gB-F)、新城疫弱毒疫苗以及传染性喉气管炎弱毒疫苗免疫和传染性喉气管炎以及新城疫强毒攻击后外周血T细胞表型亚类(CD4+、CD8+、TCRγδ+)的变化动态进行监测。重组疫苗和禽痘疫苗免疫之后,CD8+和TCRγδ+的细胞数先升高后回落;在NDV强毒攻击之后,ND疫苗免疫组的TCRγδ+数量升高,rFPV-gB-F免疫组的三种细胞数量在第1周都下降,随后升高;ILT疫苗在免疫后的第1周,CD4+细胞数量下降。结果提示rFPV-gB-F可以诱发相应的细胞免疫应答,但是有关传染性喉气管炎病毒导致的细胞免疫需要进一步的研究。The kinetic of T cell subtypes （CD4^＋, CD8^＋, TCRγδ^＋）in the periphil blood of the chickens inoculated with recombinant fowl poxvirus expressing glycoprotein B （gB） gene of infectious larngotracheitis virus （ILTV） and F gene of newcastle disease virus （NDV） （rFPV-gB-F） was studied. After inoculated with rFPV-gB-F and fowlpox virus, CD8^＋ and TCRγδ^＋cell increased in the first week then declined to normal in the second week. After challenged with NDV F48E9, CD4^＋, CD8^＋ and TCRγδ^＋ cells in the group inoculated with rFPV-gB-F decreased significantly in the first week then increased to normal level; and TCRγδ^＋ cells in ND vaccine inoculated group increased after challenge. CD4^＋ cell in ILTV vaccine inoculated group decreased in the first week then come to the normal level.

关 键 词：传染性喉气管炎 新城疫 重组禽痘病毒 外周血T淋巴细胞 

分 类 号：Q784[生物学—分子生物学] S852.65[农业科学—基础兽医学]