表达鸡γ-干扰素和传染性支气管病毒S1基因的重组鸡痘病毒疫苗对鸡外周血T淋巴细胞动态分布影响的研究  

The influence of recombinant fowlpox virus co-expressing IBV S1 gene and ChIFNγ gene on the dynamic quantitative distribution of CD4^+、CD8^+ and TCRδγ^+ T lymphocytes in SPF chickens peripheral blood

作　　者：孙永科[1] 田占成[1] 王云峰[1] 刘胜旺[1] 童光志[1] 智海东[1] 王玫[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室

出　　处：《中国预防兽医学报》2006年第5期539-543,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家"863"计划资助项目(2003AA213020)

摘　　要：机体的免疫应答是一个复杂的生物学过程,它以体液和细胞免疫为主,二者相互影响,相互作用,而构成了一个动态整体。本研究通过FACS技术检测SPF鸡接种共表达IBVS1基因和鸡γ-干扰素基因的重组鸡痘病毒疫苗后外周血中CD4+、CD8+和TCRδγ+三种亚类T淋巴细胞的数量的变化,初步探讨了干扰素对鸡外周血中T淋巴细胞亚类动态分布的影响。结果表明,含有干扰素基因的重组鸡痘病毒能显著增高机体特异性的CD8+和TCRδγ+T淋巴细胞水平,CD4+T淋巴细胞比例则要低于其他的疫苗免疫组。The immune response is a complicated process, it contains humoral immunity and cellular immunity, they affect each other and forms a dynamic system. In this study, FACS technique was used to detect the quantitative changes of CD4^＋, CD8^＋ and TCRγ^＋ T lymphocyte in SPF chickens peripheral blood after inoculating recombinant fowlpox virus which co-expressing IBV S1 gene and ChIFNγ gene, in order to discuss the interferon＇s affection on T lymphocyte dynamic distribution in peripheral blood. The results showed that the recombinant fowlpox virus can boost the maturation of CD8^＋ and TCRδγ^＋ T lymphocyte, while decrease the ratio of CD4~T lymphocyte compared to other groups.

关 键 词：T淋巴细胞 鸡γ-干扰素 传染性支气管病毒 重组鸡痘病毒 

分 类 号：S858.31[农业科学—临床兽医学] Q784[农业科学—兽医学]