茅莓黄酮液相色谱-电喷雾质谱与薄层色谱-表面增强拉曼光谱研究  

Analysis of Flavones in Rubus Parvifolius Linn by High Performance Liquid Chromatography-Electrospray Ionization Mass Spectroscopy and Thin Layer Chromatography-Fourier Transform Surface Enhanced Raman Spectroscopy

作　　者：汪瑗[1] 贺玖明[2] 陈惠[1] 张荻生[1] 才华[1] 邵会波[1] 

机构地区：[1]首都师范大学化学系,北京100037 [2]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《分析化学》2006年第8期1073-1077,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.29973026)

摘　　要：采用紫外光谱定性定量分析野生植物茅莓主要有效成分黄酮化合物,并对其进行液相色谱-电喷雾质谱联用、薄层色谱原位表面增强拉曼散射研究。液相色谱流动相A:CH3OH,B:H2O+1%乙酸,液相色谱流出时间16.21 m in的化合物为六羟基双氢黄酮醇二聚体形式,分子量640,占总黄酮的91.71%;流出时间19.61 m in的化合物分子量448,为槲皮甙,占总黄酮的8.29%。茅莓总黄酮经聚酰胺薄层色谱板分离,获得两个斑点:R f1=0.14,R f2=0.47;分别制备灰银胶和溴化银光致还原银溶胶表面增强基底,在样品薄层色谱两个斑点原位分别获得2种黄酮化合物的表面增强拉曼光谱,在2种表面增强基底作用下,都能观察到μg量黄酮化合物特征拉曼光谱,并比较了2种色谱分离与指纹检测联用技术。Qualitative and quantitative analyses for total flavones in wild Rubus parvifolius Linn （RPL） were performed using ultraviolet spectroscopy and the isolation and the structure determination of their main active ingredients were carried out by high performance liquid chromatography （HPLC）, thin layer chromatography （TLC）, coupled with electrospray ionization mass spectroscopy （ESI-MS）and Fourier transform surface enhanced Raman spectroscopy （FT-SERS）. Two flavones, herahydroxy dihydroflavanonol and quercetin in RPL were separated. Two chromatographic spots were gotten when polyamide TLC plate was used to separate the flavones,and two strengthen bases were prepared by reducing silver nitrate with trisodium citrate and photoinduced reducing the mixture of silver nitrate and potassium bromide. Strong characteristic Raman spectral bands of finger printing could be observed on these bases for microgram quantity of the flavones compound.

关 键 词：茅莓 黄酮 液相色谱-电喷雾质谱 薄层色谱 傅立叶变换表面增强拉曼光谱 

分 类 号：O657.63[理学—分析化学] O657.37[理学—化学]