在线样品浓缩毛细管区带电泳分析毛发中的苯丙胺类毒品  

Determination of Amphetamines in Hair by Capillary Zone Electrophoresis with Field-Amplified Sample Stacking

作　　者：孟品佳[1] 

机构地区：[1]中国人民公安大学刑事科学技术系,北京100038

出　　处：《分析化学》2006年第8期1137-1140,共4页Chinese Journal of Analytical Chemistry

基　　金：公安部项目(No.20049123401);北京教育委员会共建项目建设计划(No.SYS1004100436)资助

摘　　要：建立了毛细管区带电泳(CZE)的在线场放大样品堆积(FASS)方法。采用含有40%乙烯乙二醇的100 mmol/L磷酸盐二元缓冲液(pH 2.5),80%异丙醇的0.1 mmol/L磷酸样品溶液,利用缓冲体系与样品溶液体系电导率的差异,在毛细管中浓缩样品组分,对苯丙胺、甲基苯丙胺、亚甲基二氧基苯丙胺(MDA)、亚甲基二氧基甲基苯丙胺(MDMA)4种毒品进行了分离和定量测定,检测的灵敏度提高约1000倍。对于标准品的检出限可达到0.06μg/L。当样品浓度高于5μg/L时,分析的相对标准偏差在10%范围之内;用该方法对添加毒品的毛发进行了提取和测定,可检测到的添加浓度为1μg/g毛发。该方法可用于生物检材中苯丙胺类毒品的检测。An on-line concentration method of field-amplified sample stacking （FASS） by capillary zone electropheresis（CZE） was developed. With the binary buffer solution containing 100 mmol/L binary phosphate buffer solustion and 40% ethylene glycol （pH 2.5） , and the sample solution containing 80% isopropanol in 0.1 mmol/L phosphorus acid, on-line concentration of the 4 amphetamines., amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine （ MDA）, 3,4-methylenemethamphtamine （ MDM A ） was accomplished in terms of the difference of conductivity between the buffer and sample solutions. The detection sensitivity was increased up to about 1000-fold with the detection limit for standards lower than 0.06 μg/L. The relative standard deviation （RSD） was about 10% when the concentrations were above 5 μg/L. The method was used to spiked hair with the level of 1 μg/g detectable, which promised the perspective application in detecting trace amount amphetamines in human hair and body fluid.

关 键 词：毛细管区带电泳 在线场放大样品堆积 苯丙胺类毒品 

分 类 号：D919.2[医药卫生—法医学]