人血浆中盐酸莫雷西嗪的高效液相色谱法测定及其药物动力学研究  

Determination of moricizine hydrochloride in human plasma by HPLC and its pharmacokinetics

作　　者：林建阳[1] 阚周密[1] 

机构地区：[1]中国医科大学附属第一医院,110001

出　　处：《山西医药杂志》2006年第9期771-773,共3页Shanxi Medical Journal

基　　金：辽宁省"十一五"教育科学规划立项基金资助项目(46-25)

摘　　要：目的 建立测定人血浆中莫雷西嗪浓度的高效液相色谱法。方法 色谱柱：HypersilODS-2（150nm×4．6mm，5μm）；流动相：甲醇：水：三乙胺（70：30：0．4），v／v／v，pH6．8）；检测波长：268nm；以氯氮平作为内标，测定人血浆中的莫雷西嗪药物浓度。结果 莫雷西嗪浓度线性范围为0．04～4mg／L，回归方程Y=11．42X＋1．441，r=0．9992；回收率96、1％，日内和日间精密度（RSD）分别为4．7％和5．3％，整个测定时间8min左右。结论 该方法简便、快速、准确，适用于临床上莫雷西嗪药物动力学的研究。Objective To estabish a HPLC method for determining the concentration of moricizine in human plasma. Methods The plasma concentrations of moricizine were determined by HPLC method. The assay was performed on the Hypersil ODS-2 column with the mobile phase consisted of methanol-water-triethylamine （70：30：0.4, v/v/v, pH 6.8）. The UV detector wavelength was set at 268 nm,and the clozapine was taken as the internal standard. Results The calibration curve was linear in the range of 0.04 ～ 4 mg/L with a regressive equation of Y=11.42 X ＋ 1.441, r = 0. 999 2. The recovery of moricizine was 96.1% ; the relative standard deviations were 4.7 % （intra day） and 5.3 % （inter day） respectively. Conclusion The method is shown to be, conveniem, accurate, rapid,and suitable for detemlining moricizine in the human plasma and pharmacokinetic studies of moricizine.

关 键 词：莫甫西嗪 色谱法 高压液相 血药浓度 

分 类 号：R96[医药卫生—药理学]