参附对脐静脉内皮细胞Toll样受体4/NF-κB途径的影响  

Effects of Shenfu injection on lipopolysaccharide-induced Toll-like receptor-(NF-κB) signal in human umbilical vein endothelial cells

作　　者：张弛[1] 范建军[1] 

机构地区：[1]山西省人民医院,030012

出　　处：《山西医药杂志》2006年第9期777-779,共3页Shanxi Medical Journal

摘　　要：目的观察参附注射液对内毒素介导的人脐静脉细胞Toll样受体4/NF-κB途径及细胞间黏附分子、E-选择素表达的影响。方法健康产妇分娩后的新鲜婴儿脐带,应用胶原酶消化,收集内皮细胞进行培养。实验分为对照组、内毒素组、内毒素+参附组。对照组:M199培养基中不加任何物质。内毒素组:M199培养基中加入脂多糖1 mg/L。内毒素+参附组:预先加入参附注射液10 mL/L,30 min后加入脂多糖1 mg/L。干预1h收集细胞,提取核蛋白测定NF-κB活性,干预12 h后收集细胞检测Toll样受体4、细胞间黏附分子1、E-选择素表达水平。结果内毒素组的Toll样受体4、细胞间黏附分子1、E-选择素表达水平、NF-κB活性均显著高于对照组,内毒素+参附组的Toll样受体4、细胞间黏附分子1、E-选择素表达水平、NF-κB活性均明显低于内毒素组。结论参附注射液可以通过抑制NF-κB活化,减弱Toll样受体4/NF-κB途径,抑制了脂多糖介导的黏附分子表达,从而减轻机体的炎症反应。Objective To investigate the effects of Shenfu injection on lipopolyccharide （LPS）-induced ＂Toll like receptor-nuclear factor kappa B （TLR4/NF-κB） signal and intercellular adhesion molecule-1 （ ICAM-1 ）, E-selection in human umbilical vein endothelial cells（HUVECs）. Methods HUVECs obtained from collagenase-digested umbilical veins were cultured and divided into 3 group： ①control group： cuhured in M199 medium without any intervention. ② LPS group： cultured in M199 medium with LPS（ 1 mg/L.）. ③LPS＋ Shenfu group： pretreated with Shenfu injection for 30min before LPS incubation. HUVECs were stimulated for 1 h, and NF-κB P65 activation were examined. HUVECs were stimulated for 12 h and then collected, TLR4, ICAM-1, and E-selection were examined. Results NF-κB P65 activity, TI.R4, ICAM- 1, E-selection mRNA and protein expression were higher in I.PS group than in control group. NF-κB P65 activity,TLR4, ICAM-1 and E-selection mRNA and protein expression were lower in LPS＋ Shenfu group than in LPS group. Conclusion Shenfu injection play a role in inhibiting adhesion molecule up-regulation and inflammatory reaction by inhibiting NF-κB activation, and subsequently suppression TLR4ANF-κB signal.

关 键 词：NF-ΚB 脂多糖类 胞间黏附分子1 E选择素 参附注射液 

分 类 号：R285[医药卫生—中药学]