VAMP-2在鲈鱼组织中的表达分布  

Expression and Distribution of VAMP-2 in Tissues of Japanese Sea Perch

作　　者：柴迎梅[1,2,3] 刘胜浩[1,2] 丛柏林[1,2] 黄晓航[1,2] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]海洋生物活性物质国家海洋局重点实验室,山东青岛266061 [3]山东大学威海分校海洋生命学院,山东威海264209

出　　处：《海洋科学进展》2005年第B12期6-10,共5页Advances in Marine Science

基　　金：国家高技术研究发展计划项目--重要海洋经济鱼类SNARE基因的克隆和表达（2002AA629120）

摘　　要：VAMP-2是真核生物中的一种膜受体蛋白，该蛋白在真核生物的胞吐、信号传导、胞内运输和分泌等活动中起到重要作用。采用免疫印迹方法和原位杂交技术，研究了VAMP-2在鲈鱼组织中的表达情况，分别对鲈鱼8种组织中的VAMP-2进行了检测。结果表明，除脑组织外，在肠、肝脏、肾脏、皮肤、脾脏、性腺等组织中也检测到VAMP-2的表达，各组织间的表达水平亦存在差异，肌肉组织未检测到表达。研究表明，鲈鱼的组织中表达的VAMP-2蛋白可能广泛参与鲈鱼的各种生理活动，并影响其生长、发育、繁殖和防卫等各项重要的生物学功能。VAMP-2 is a membrane receptor protein widely distributed in eukaryote and plays an important role in exocytosis, signal transduction, intracellular traffic and secretion. The Western blot and in-situ hybridization were used to study the expressions of VAMP-2 in different tissues of Japanese sea perch. It is shown from the detection results that VAMP-2 is expressed in intestine, liver, kidney, skin, spleen and sex gland as well as brain of Japanese sea perch at different levels, but no VAMP-2 is expressed in muscle, which indicates that VAMP-2 may widely involve in various physiologcial activities of Japanese sea perch and affect its important biofunctions such as growth, development, reproduction and defense .

关 键 词：SNARE蛋白 免疫印迹 原位杂交 

分 类 号：Q959.46[生物学—动物学]