南极冰藻谷胱甘肽相关因子的测定  

Determination of Glutathione-related Factors of Antarctic Ice Microalgae

作　　者：丁燏[1] 缪锦来[2] 王全富[3] 李光友[2] 简纪常[1] 吴灶和[1] 

机构地区：[1]广东海洋大学海洋水产经济动物病原生物学及流行病学省级重点实验室,广东湛江524025 [2]海洋生物活性物质国家海洋局重点实验室,山东青岛266061 [3]哈尔滨工业大学威海海洋学院,山东威海264209

出　　处：《海洋科学进展》2005年第B12期24-29,共6页Advances in Marine Science

基　　金：国家自然科学基金项目--南极冰藻活性物质的研究（40506005）

摘　　要：谷胱甘肽系统在清除活性氧和生物保护中发挥重要作用，探讨了南极冰藻胞内谷胱甘肽含量及谷胱甘肽相关酶的活力。采用分光光度法，对24种南极冰藻胞内谷胱甘肽含量、谷胱甘肽合成能力（GPA）、谷胱甘肽还原酶活力等进行测定。测定结果表明，南极蓝藻B-1中谷胱甘肽含量最高；南极衣藻ICE-L和南极硅藻GJ01的谷胱甘肽总产量居前2位；南极冰藻GPA普遍高于常温藻的。南极硅藻GJ01和南极衣藻ICE—L GR活力高于对照组的。培养基的选择表明，f／2培养基适合南极硅藻GJ01的生长，而Provasoli培养基适合南极衣藻ICE—L的生长。可见，南极冰藻成为谷胱甘肽的新来源是有可能的，尤其是南极硅藻GJ01和南极衣藻ICE—L。Glutathione （GSH） and GSH-related enzymes play an important role in protecting organisms from damage made by reactive oxygen. In this paper, the GSH content and GSH-related enzyme activities in Antactic ice microalgae are deiscussed. The spectrophotometric method was used to determine the GSH contents, glutathione produce ability （GPA） and glutathione reductase （GR） activities in 24 species of Antactic ice microalgae, and it is shown from the determination results that the GSH content in Antarctic Cyanophyceae B-1 is the highest, the total GSH products of Antarctic Chlamydomonas sp. ICE-L and Berkeleya rutilans GJ01 rank in the first two places, and the GPAs of Antarctic Chlamydomas sp. ICE-L and Berkeleya rutilans GJ01 are higher than those in the control microalgae. The GR activities in Antarctic Chlamydomas sp. ICE-L and Berkeleya rutilans GJ01 f/2 culture medium is more suitable for the growth of are higher than those in the control microalgae. The B. rutilans GJ01, and the Provasoli culture medium is more suitable for the growth of Chlamydomas sp. ICE-L. It is shown from the study results that Antac tic ice microalgae, especially Chlamydomas sp. ICE-L and B. rutilans GJ01 can be a new source of GSH.

关 键 词：谷胱甘肽 南极冰藻 谷胱甘肽合成能力 谷胱甘肽还原酶 

分 类 号：Q94[生物学—植物学]