白藜芦醇的电化学行为及其与DNA的相互作用  

Electrochemical Behavior of Resveratrol and Its Interaction with DNA

作　　者：郑建斌[1] 张宏芳[1] 张秀琦[1] 高鸿[1] 

机构地区：[1]西北大学分析科学研究所

出　　处：《高等学校化学学报》2006年第9期1635-1639,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20275030);陕西省自然科学基金(批准号:2004B20)资助

摘　　要：采用电化学方法研究了白藜芦醇在pH=2～13的缓冲溶液中的电化学行为、抗氧化能力及其与DNA的相互作用．研究结果表明，在2．0〈pH〈7．1的介质中，白藜芦醇产生的P1波是其中性分子的2e，2H^＋不可逆还原波；在7．3〈pH〈10．9的溶液中，白藜芦醇产生的P3波与P1波具有相同的还原机理，在较正的电位下白藜芦醇产生的P2波是其一价阴离子的可逆2e，2H^＋还原波；在pH〉11的溶液中，白藜芦醇产生的P4和P5波分别是其二价和三价阴离子的还原波．在最佳实验条件下，微分脉冲极谱图上Ip3在8．0×10^-8～2．0×10^-6mol／L范围内与白藜芦醇的浓度呈线性关系，检出限为4×10^-8mol／L．将该法用于中药虎杖中白藜芦醇含量的测定，结果与高效液相色谱法一致．Electrochemical techniques were employed to study the electrochemical behavior in a wider pH range, the antioxidative ability and the interaction with DNA of resveratrol. The experimental results show that wave P1 detected in 2.0 〈 pH 〈 7.1 solutions was an irreversible reduction wave of resveratrol involving 2e and 2H ^＋ ; and wave P3 detected in 7.3 〈 pH 〈 10.9 solutions was caused by the same reason with P1, wave P2 located at less negative potential was produced by the reversible reduction of ionized resveratrol involving 2e and 2H ^＋ ; as expected, the reduction waves P4 and P5 of bivalent and trivalent anion of further ionized resveratrol also present in the solution with pH 〉 11. Under the optimum conditions, a linear relationship could be established between the peak current of wave P3 and the concentration of resveratrol in the range from 8.0 × 10 ^-8 to 2.0 × 10^-6 moL/L, and the detection limit was 4 × 10^-8 moL/L. The determination result of content of resveratrol in Chinese traditional herbal medicine Polygonum cuspidatum by the proposed differential pulse polarography method was consistent with the result by the comparison HPLC method. The antioxidative ability of resveratrol and the interaction mode of resveratrol with DNA were proved by electrochemical method.

关 键 词：电化学行为 白藜芦醇 还原波 虎杖 DNA 

分 类 号：O657[理学—分析化学]