Co@SiO_2核壳式纳米磁性粒子的合成、性质表征及在细胞分离和细胞芯片上的应用  

Synthesis Property Characterization of Core-shell Co@SiO_2 Magnetic Nanoparticle and Its Biomedical Applications to Cell Separation and Cell Chip

作　　者：郭小英[1] 王永宁[2] 顾林岗[3] 贺艳峰[4] 张春秀[1] 唐祖明[1] 陆祖宏[1] 

机构地区：[1]东南大学生物科学与医学工程系 [2]中国科学院理化技术研究所,北京100080 [3]江苏省公安厅物证鉴定中心,南京210024 [4]东南大学公共卫生学院,南京210009

出　　处：《高等学校化学学报》2006年第9期1725-1728,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:60121101;60223002);国家高新技术研究发展计划项目(批准号:2002AA2Z2041;2004AA302070)资助

摘　　要：合成了Co@SiO2核壳式纳米粒子,并采用透射电镜(TEM)、X射线衍射(XRD)、扫描电镜(SEM)和振动样品磁强计(VSM)对其形状、尺寸、荧光及磁特性进行了表征,探讨了其在细胞分离和细胞芯片上的应用和原理.In this paper, Co@ SiO2 nanoparticles with the apparent core-shell magnetic nanostructure were synthesized by reducing and hydrolysis in aqueous solutions. The TEM, VSM, SEM, and the confocal fluorescence microscope were utilized to characterize the morphology, size, magnetism of the Co @ SiO2 nanoparticles, and the core-shell materials were applied to the cell separation, immunophenotyping of leukemias and lymphomas diagnostics chip, in which strenuous procedures were omitted by the use of the magnetic core-shell nanoparticles, and separation. These core-shell nanoparticles were proved to have obvious cores and shell, and the cores have strong magnetism, and the shell protected these nanosized metal particles from coagulation due to anistropic dipolar attraction and oxidation very well. However this method could not limited the original cores below the size of 10 nm, which is the critical size of superparamagnetisizm, as a result coagulation due to remanent magnetism limited this core-shell particles from further applications in bioseparation.

关 键 词：核壳式 纳米磁珠 细胞芯片 细胞分离 

分 类 号：O647.2[理学—物理化学]