房颤心房肌L型钙通道α1c亚单位mRNA的表达及意义  

Gene expression of L-type Ca^(2+)-channel alpha 1c subunit in atrial myocardium of chronic auricular fibrillation patients

作　　者：曾祥君[1] 肖颖彬[1] 钟前进[1] 陈林[1] 王学锋[1] 

机构地区：[1]第三军医大学新桥医院,重庆400037

出　　处：《山东医药》2006年第25期11-12,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30370583)

摘　　要：目的研究房颤心房肌L型钙通道结构重塑的分子基础。方法心脏手术中采集慢性房颤及窦性心律患者的右心房肌,提取总RNA行逆转录,用PCR技术检测L型钙通道α1c亚单位不同位置的mRNA片段表达。结果①α1c亚单位Ⅰ~Ⅱ跨膜区连接对应的mRNA丰度在慢性房颤心房肌与窦性心律心房肌无明显差异。②α1c亚单位Ⅳ跨膜区对应的mRNA丰度则慢性房颤心房肌较窦性心律心房肌明显降低。结论房颤心房肌L型钙通道结构重塑与α1c亚单位的亚型表达改变有关。[Objective]To investigate the molecular mechanisms of atrial electrical remodeling in chronic auricular fibrillation （AF） patients, and the gene expression of L-type Ca^2＋-channel α1c subunits in atrial myocardium of AF patients. [Methods] Right atrial myocardium were obtained from 24 patients with chronic AF and 19 controls in sinus rhythm. All patients underwent valve replacement surgery. Total RNA was isolated and reversely transcribed into cDNA. cDNA of interest and glyceraldehyde-3-phosphate dehydrogenase were amplified by semi-quantitative polymerase chain reaction, and separated by ethidium bromide-stained gel electrophoresis. [Results] mRNA expression corresponding to the Ⅰ - Ⅱ link of α1c subunits did not altered in patients with chronic AF compared to the controls,while that corresponding to the Ⅳ trans-membrane of α1c subunits was reduced. [Conclusion] Atrial myocardium L-type Ca^2＋-channel remodeling in patients with atrial fibrillation may be due to down-regulated expression of the isoform of α1c subunits.

关 键 词：房颤 L型钙通道 重构 

分 类 号：R541.7[医药卫生—心血管疾病]