山东地区新生儿脐血HLA-A、HLA-B等位基因多态性研究  

Study of HLA-A and HLA-B allele polymorphism in Shandong neonates umbilical cord blood

作　　者：戴云鹏[1] 沈柏均 阎文英[3] 王红美[1] 

机构地区：[1]山东省立医院,山东济南250021 [2]山东脐血造血干细胞库 [3]山东大学临床医学院

出　　处：《山东医药》2006年第25期19-21,共3页Shandong Medical Journal

基　　金：山东省卫生厅青年科学基金项目(2005026)

摘　　要：目的研究山东地区新生儿脐血人类白细胞抗原(HLA-A、HLA-B)等位基因的分布特征,探讨国人用山东脐血供者进行造血干细胞移植的可能性。方法应用PCR-SSO方法对山东地区5 844例无血缘关系的汉族健康新生儿脐血进行HLA-A、HLA-B等位基因分布调查。结果检出20种HLA-A等位基因,频率较高的为A*02(0.3041)、A*11(0.1443)、A*24(0.1434)、A*30(0.0975)和A*33(0.0859),较低的为A*34(0.0006)、A*25(0.0005)、A*66(0.0005)、A*74(0.0004)和A*36(0.0001)。检出46种HLA-B等位基因,频率较高的为B*13(0.1348)、B*51(0.0713)、B*62(0.0712)、B*61(0.0676)和B*60(0.0642),较低的为B*77(0.0001),B*76(0.0002),B*47(0.0003),B*42(0.0003)和B*72(0.0004)。结论山东新生儿脐血HLA I类基因具有多态性,能代表山东汉族人群HLA的分布特征,反映北方汉族HLA的分布规律;国人(尤其是北方汉族人)在山东脐血库中最容易找到HLA I类等位基因相合的异基因脐血供者。[Objective] To investigate the HLA-A,-B allele polymorphism in Shandong neonates umbilical cord blood and explore the possibility of finding the cord blood donor of HLA-A,-B matched to perform the stem cell transplantation for Chinese. [Methods] The subjects of the study were drawn from 5844Shandong unrelated umbilical cord blood samples in Shandong Umbilical Cord Bank and investigated by polymerase chain reactionsequence specific oligo-nucleotide （PCR-SSO）. [ Results] 20 alleles at HLA-A locus, and 46 alleles at HLA-B locus in Shandong umbilical cord samples were detected. Among 20 HLA-A alleles detected, the most frequent five alleles were A ＊ 02（0. 3041）, A ＊ 11（0. 1443）, A ＊ 24（0. 1434）, A ＊ 30（0. 0975） and A ＊ 33（0. 0859）. A 34 （0. 0006）, A ＊ 25（0. 0005, A ＊ 66（0. 0005）, A ＊ 74（0. 0004） and A ＊ 36（0. 0001） had lower gene frequency. Among 46 HLA-B alleles detected, the most frequent five alleles were B ＊ 13（0. 1348）, B ＊ 51（0. 0713）, B ＊ 62 （0. 0712）, B＊61（0. 0676）and B~ 60（0. 0642） ; HLA-B＊ 77（0.0001）, B＊ 76（0. 0002）, B＊ 47（0. 0003）, B ＊ 42 （0. 0003）and B ＊ 72 （0. 0004） showed lower gene frequency. [Conclusion] HLA-A,-B allele in Shandong umbilical cord blood have rich polymorphism, the distributing character of HLA-A,-B allele in Shandong Hans were showed, and the HLA distributing character in Northern Chinese was further reflected. Chinese patients are easier to search HLA-A, -B matched cord blood donors in Shandong Umbilical Cord Blood Bank, especially Northern Han Chinese.

关 键 词：脐血 人白细胞抗原 基因多态性 

分 类 号：R457[医药卫生—治疗学] R714.56[医药卫生—临床医学]