煤和液化石油气燃烧颗粒物对YG菌株的致突变活性研究  

Mutagenicity of Particulates Produced by Coal and Liquefied Petroleum Gas Combustion in YG Strains

作　　者：阎洪涛[1] 刘君卓[2] 孔祥环[3] 尹学钧[3] 肖中新[2] 

机构地区：[1]承德医学院卫生学教研室,067000 [2]北京医科大学环境卫生教研室 [3]首都医科大学环境卫生教研室

出　　处：《环境与健康杂志》1996年第3期100-102,共3页Journal of Environment and Health

基　　金：本课题得到国家自然科学基金资助

摘　　要：应用日本新构建的对硝基多环芳烃具有高敏感性的鼠伤寒沙门氏菌株 YG1021 和 YG1024,对北京市民用蜂窝煤和液化石油气燃烧颗粒物的丙酮提取物进行了致突变研究。结果显示,两种提取物均具有明显的间接和直接致突变作用。表明颗粒物中不仅含有多环芳烃类的间接致突变物,而且还含有直接致突变的硝基多环芳烃。从单位重量提取物的所致回变菌落数及出现阳性结果的最低剂量可见,液化石油气燃烧颗粒物的致突变活性明显高于燃煤颗粒物;YG 菌株更适用于环境样品中硝基多环芳烃的致突变活性检测。The mutagenicity of acetone extracts of coal and liquefied petroleum gas (LPG) combustion particulates was studied with Salmonella typhimurium strain TA98 and two of its new constructed derivatives,i.e.YG1021,a nitroreductase- overproducing derivative,and YG1024,with higher levels of O-acetyhransferase.The results showed that acetone extracts of the two particulates were direct or indirect significant mutagens in 3 strains.These suggested that the particulates contain not only some indirect mutagenic polyaromatic hydrocarbons (PAHs),but also direct mutagenic nitrated polyaromatic hydrocarbons (NO_2-PAHs).By comparing the mutagenicity of extracts of both combustion particulates per weight,LPG was found to be more mutagenic than coal. When detecting the mutagenicity of NO_2-PAHs,YG strains were more sensitive than TA98,which had been classically belived sensitive strain to this class of mutagens.

关 键 词：煤 液化石油气 致突变试验 硝基多环芳烃 

分 类 号：X513.031[环境科学与工程—环境工程]