中华眼镜蛇毒组分CⅡ-3-1对多药耐药K562/A02细胞株作用研究  

Study on Effect of Multidrug Resistance in K562/A02 Cell Line by Component CⅡ-3-1 from CⅡ Naja naja atra(Chinese Cobra) Venom

作　　者：成友军[1] 孔天翰[1] 董伟华[2] 

机构地区：[1]广州医学院蛇毒研究所,广东广州510182 [2]广州医学院病理生理学教研室,广东广州510182

出　　处：《蛇志》2006年第3期173-177,共5页Journal of Snake

基　　金：广州市科技局资助项目(穗科条字[2005]6号)。

摘　　要：目的从眼镜蛇毒组分CⅡ中寻找抗肿瘤有效组分并研究其对多药耐药K562/A02细胞的杀伤作用。方法用SephadexG-75、SephadexG-25层析法从蛇毒中分离、纯化抗肿瘤有效组分,经SDS-PAGE电泳、HPLC分析、鉴定,用MTT法观察细胞毒性作用。结果CⅡ-3-1(近似电泳纯)的LD50为(0.622±0.007)mg/kg。CⅡ-3-1对耐药K562/A02及敏感K562细胞均有抑制作用。结论中华眼镜蛇毒中组分CⅡ-3-1对K562及K562/A02细胞都有显著杀伤作用。Objective To discover the antineoplastic component C Ⅱ-3-1 of Naja naja atra （Chinese cobra） Venom and observe its effect on Multidrug-resistant human leukemia cell line K562/A02. Methods We isolate and purify C Ⅱ-3-1 from chinese cobra venom by G- 75 and G-25 MS chromatography assays, analyze and identify by SDS electrophoresis and HPLC assays, study its toxic action in human leukemia cells by MTT assays. Results The LD50 of Component CⅡ-3-1 was （0. 622±0. 007）mg/kg. The rate of K562 and K562/ A02 cell growth was obviously inhibited by Component C Ⅱ-3-1; Conclusion The rate of K562 and K562/A02 cell growth was obviously inhibited by Component C Ⅱ-3-1.

关 键 词：眼镜蛇 眼镜蛇毒 多药耐药 人白血病细胞 抗肿瘤药 

分 类 号：R996.3[医药卫生—毒理学]