骨髓单个核细胞移植和冠状动脉介入治疗后再狭窄关系的实验研究  

Experimental study on coronary restenosis after bone marrow mononuclear cells transplantation

作　　者：温尚煜[1] 毛节明[1] 徐晓蓉[1] 李海燕[1] 潘震华[1] 郭艳红[1] 周春燕[2] 高炜[1] 

机构地区：[1]北京大学第三医院心内科北京大学心血管研究所,博士研究生100083 [2]北京大学医学部生化与分子生物学系

出　　处：《中国介入心脏病学杂志》2006年第4期237-240,共4页Chinese Journal of Interventional Cardiology

基　　金："十五"国家科技攻关计划项目(2004BA714B-1)

摘　　要：目的研究骨髓单个核细胞移植对冠状动脉介入治疗后支架内再狭窄的影响。方法用球囊堵闭法制成小型猪急性前壁心肌梗死模型,喂养3周后在左前降支置入支架,同时提取骨髓分离单个核细胞,注入梗死相关血管。饲养4周后做冠状动脉造影定量分析狭窄程度。对支架两端血管组织学切片做苏木精-伊红和天狼猩红染色分析再狭窄原因。结果实验组8头小型猪中有4头发生再狭窄,再狭窄率为50%;对照组9头中有4头发生再狭窄,再狭窄率为44%,两组间比较差异无统计学意义;冠状动脉造影定量分析提示:骨髓单个核细胞治疗组冠状动脉支架内管腔晚期丢失为1·50±1·45mm,对照组为1·31±1·07mm(P=0·736),两组比较差异无统计学意义。结论在小型猪心肌缺血模型中,骨髓单个核干细胞移植不增加冠状动脉介入治疗后支架内再狭窄发生率。Objective To study the effect of bone marrow mononuclear cells transplantation on restenosis rate and its mechanism. Methods The left anterior descending coronary arteries of swines were obstructed by balloon to create myocardial infarction models. After 3 weeks coronary stents were implanted at the middle part of the left anterior descending coronary arteries followed by the injection of bone marrow mononuclear cells into the coronary arteries. The degree of restenesis were measure by quantitative coronary angiography （QCA） after four weeks. Vascular tissue at both ends of stents were tested by HE and Sirius staining to analyse the mechanism of restenosis Results At end of the experiment there were 8 swines in the bone marrow rnononuclear cells transplantation group and 9 in the control group. After injection of bone marrow mononuclear cells the restenosis rate was similar to the control group （50% vs 44%, P = 0.762）. The lumen late loss was also similar between the two groups （1.50 ± 1.45 mm vs 1.31 ± 1.07 mm, P = 0.736）. Condusion Bone marrow mononuelear cells transplantation does not increase the restenosis rate after percutaneous coronary artery intervention.

关 键 词：心肌梗死 骨髓细胞 细胞移植 再狭窄 

分 类 号：R654.2[医药卫生—外科学]