肾性高血压大鼠主动脉血红素加氧酶-1表达增加  

Increase of heme oxygenase expression in the aorta of renal hypertensive rats

作　　者：庞东卫[1] 徐海[2] 刘俊昌[2] 潘桂兰[1] 韩莉[2] 

机构地区：[1]包头医学院生理教研室,内蒙古包头014010 [2]北京大学医学部生理与病理生理学系,北京100083

出　　处：《基础医学与临床》2006年第8期868-870,共3页Basic and Clinical Medicine

摘　　要：目的探讨肾性高血压时血红素加氧酶(HO)/一氧化碳(CO)系统的变化,以明确血红素加氧酶在肾性高血压发病过程中的意义及作用。方法①制作两肾一夹(2K1C)肾性高血压大鼠模型,分别于2、4、6、8周记录血压,用放免法测定血浆中血管紧张素II的含量。②分光光度比色法测定主动脉微粒体中HO的活性。③用W estern b lot方法测定主动脉HO-1蛋白的表达。结果从术后2周起,2K1C组血压明显高于假手术组(P<0.01);同时血浆AngII含量显著增加(P<0.01)。2K1C组在4周和6周时主动脉HO活性升高明显(P<0.05),但8周时有所回降,但仍高于对照组;2K1C组4周时HO-1蛋白含量明显升高,比假手术组高25.38%(P<0.05)。结论在肾性高血压大鼠,血浆AngII增高诱导了HO-1的表达增加来对抗压力负荷对血管的损害。Objective To investigate the role of heme oxygenase / carbon monoxide （ HO/CO） pathway in the development of renal hypertension. Methods （ 1 ） The animal model of two-kidney-one clip renal hypertensive rats were prepared and the plasma contents of angiotensin Ⅱ （Ang Ⅱ ） were determined by radiommunoassay. （2）The activity of HO was tested at 2, 4, 6, 8 weeks after surgery. （3） Western blot was used to estimated the level of HO-1 expression of aorta. Results In 2K1C rats, there was a significant increase in blood pressure at 2K1C. At the same time, rennin-agiotensin system （RAS） in plasma was activated and the content of Ang Ⅱ obviously increased. The activity of HO was greater at 4- and 6-week in 2K1C group than sham group（P 〈 0. 05）. However, the activity of HO decreased at 8-week in 2K1C group. Western blot showed that the content of HO-1 protein in aorta of 2K1C group was increased as compared with sham group （ P 〈 0. 05 ）. Conclusions In renal hypertension, the increased content of Ang Ⅱinduced HO-1 expression to compensate the damage on blood vessels.

关 键 词：血红素加氧酶 一氧化碳 肾性高血压 血管紧张素Ⅱ 

分 类 号：R544.14[医药卫生—心血管疾病]