Cu^(2+)污染土壤接种AM真菌对紫云英生长的影响  

Effect of inoculation of arbuscular mycorrhizal fungi on the growth of Astragalus sinicus L. in copper ion polluted soil

作　　者：陈秀华[1] 赵斌[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室

出　　处：《菌物学报》2006年第3期416-423,共8页Mycosystema

基　　金：国家自然科学基金(30270051);湖北省国际合作重点项目(2003CA020)资助

摘　　要：通过5个土壤Cu2+水平(0,20,50,100,150mgkg-1)的盆栽试验,研究了不同土壤Cu2+水平接种AM真菌对紫云英生长的影响。结果表明:(1)随着土壤Cu2+水平升高,紫云英生物量下降,与未接种相比,接种AM真菌明显提高了紫云英的生物量,接种G.intraradices对紫云英生物量的提高比接种G.mosseae更为明显,两者间呈显著性差异。(2)随着土壤Cu2+水平升高,紫云英根段浸染率下降,菌丝琥珀酸脱氢酶、碱性磷酸酶活性也下降。(3)在相同土壤Cu2+水平接种不同的AM真菌,紫云英根段浸染率有显著差异,接种G.intraradices的紫云英根段浸染率显著高于接种G.mosseae的处理,其菌丝琥珀酸脱氢酶活性及碱性磷酸酶活性也显著高于接种G.mosseae的处理。(4)接种G.intraradices能显著抑制Cu2+从紫云英地下部分向地上部分的运转,降低Cu2+的毒害,接种G.mosseae相对促进了Cu2+的运转。以上结果显示,Cu2+污染土壤中接种G.intraradices对紫云英生长具有促进作用。In a pot experiment, the effect of inoculation of arbuscular mycorrhiza （AM） fungi on Astragalus sinicus in copper polluted soil was studied. Associations were established between Astragalus sinicus and two species of AM fimgi, Glomus intraradices and G mosseae. The result showed that copper inhibited the development of AM fungi. The colonization rate of AM fungi was decreased with the increase of copper added in soil and the enzyme activity of succinate dehydrogenase （SDH） and alkaline phosphatase （ALP） were decreased also. Moreover, the biomass of plants decreased with the increase of copper added in soil, but the biomass of plants inoculated with AM fungi was higher than that of non-inoculated plants. Furthermore, the biomass of plants inoculated with G intraradices was higher than that of plants inoculated with G mosseae. Inoculation of G intraradices lowered copper transferring to the shoots, but inoculation of G mosseae accelerated the transference of copper. It was concluded that inoculation with G intraradices enhanced the resistance ofAstragalus sinicus to copper polluted soil.

关 键 词：浸染率 琥珀酸脱氢酶活性 碱性磷酸酶活性 生物量 

分 类 号：X53[环境科学与工程—环境工程]