瘦素对人类外周血T淋巴细胞增殖作用的影响  

The effect of Leptin on the proliferation of human circulating T lymphocytes

作　　者：富东旭[1] 李大林[1] 李殿俊[1] 李霞[1] 付东然[2] 李波[1] 

机构地区：[1]哈尔滨医科大学免疫教研室,哈尔滨150086 [2]哈尔滨医科大学第二临床医学院皮肤科,哈尔滨150086

出　　处：《中国免疫学杂志》2006年第7期604-606,611,共4页Chinese Journal of Immunology

基　　金：黑龙江省科委攻关项目资助(GB05C402-03)

摘　　要：目的:探讨瘦素(Leptin)对人外周血T淋巴细胞增殖作用的影响。方法:分离并培养人外周血T淋巴细胞,采用[3H]TdR掺入试验方法,观察不同浓度Leptin的单独作用及其与PHA的协同作用对人外周血T淋巴细胞增殖作用的影响。结果:不同浓度Leptin的单独作用对人类外周血T淋巴细胞均无刺激增殖作用,但可协同PHA的刺激增殖作用,并在一定浓度范围内呈现剂量依赖性。结论:瘦素可协同PHA刺激人类外周血T淋巴细胞增殖。Objective：To investigate the effect of human Leptin on the proliferation of human circulating T lymphocytes in vitro and the influence of Leptin on the action of PHA on the proliferation of human circulating T lymphocytes. Methods ： PBMC were isolated from hepartinized venous blood of normal donors by density-gradient sedimentation over ficoll-Hypaque and cultured in the 1640 complete medium. T lymphocytes were then obtained by depleting the monocyte and B cell populations. Cells were then cultured in the 1640 complete medium for 56 h in the presence of PHA. The proliferative effect was assessed by means of incorporation of [ ^3 H ] thymidine. [ ^3 H ] TdR was added and cultured for 16 hours. Cells were then processed in the harvester to measure the incorporation of [ ^3 H ] thymidine. Radioactivity was determined by liquid scintillation counter. Results：The results showed that Leptin alone did not affect the proliferation of T lymphocytes, hut it could enhance the effect of PHA on the proliferation of T lymphocytes at the concentrations of PHA from 2-8 μg/ml. A dose-response studies of T lymphocyte proliferation showed that the maximal effect of Leptin were observed at 10 nmol/L. Leptin did not affect the proliferation of T lymphocytes when the concentrations of PHA were over 8 μg/ml. Conclusion：The studies demonstrated that Leptin alone did not affect the proliferation of lymphocytes, hut it could enhance the action of PHA on cell growth in a dose-dependent manner.

关 键 词：瘦素 T淋巴细胞 [^3H]TdR 淋巴细胞增殖 

分 类 号：R392.11[医药卫生—免疫学]