钙离子载体上调K562细胞B7分子表达  

Upregulation of B7 molecules expression by calcium ionorphore in K562 cells in vitro

作　　者：郜峰[1] 陈君敏[1] 叶德富[1] 林寿榕[2] 

机构地区：[1]福建医科大学附属第一医院血液科,福州350005 [2]福建医科大学附属第一医院检验科,福州350005

出　　处：《中国免疫学杂志》2006年第7期612-614,618,共4页Chinese Journal of Immunology

基　　金：福建省自然科学基金资助项目(C0210014)

摘　　要：目的:探讨钙离子载体(Calc ium ionorphore,C I)对慢性髓系白血病细胞株K562细胞表面B7分子表达的上调作用。方法:将生长状态良好的K562细胞在含C I(375 ng/m l)的培养基中培养,以不含C I培养的K562细胞作对照组。培养0、48和96小时后台盼蓝拒染法检测细胞数和细胞存活率,流式细胞仪检测培养前及培养96小时后细胞表面B7分子的表达情况,MTT比色法检测其刺激同种异体T细胞增殖的作用。结果:K562细胞表面B7分子缺如或低表达;用含C I的培养基培养96小时后,可显著上调B7分子的表达,且能明显激活同种异体T细胞。培养前后细胞形态无明显变化,加C I培养的K562细胞较不加C I培养的细胞生长缓慢。结论:慢性髓系白血病细胞株K562细胞表面存在B7分子表达缺陷,C I可上调其B7分子。C I可能有抑制K562细胞生长的作用。Objective：To investigate the effect of calcium ionorphore on B7 costimulatory molecules of chronic myeloid leukemia cells line K562. Methods：Well growing K562 cells were cultured in the medium containing calcium ionorphore（375 ng/ml）, with K562 cells without CI treatment as control. The cells＇ viability and number were calculated by Trypan Blue exclusion at 0,48 and 96 h. Before and after 96 h of cultured, BT-1 and B7-2 expression was assayed by flow cytometry. The proliferation of allogeneic human T cells was measured by MTT colorimetry. Results： B7 costimulatory molecules were abcent or lowly expressed on K562 cells. After 96 h of CI treatment, B7 costimulatory molecules of K562 cells were markedly up-regulated and marked activation of allogeneic T cells occurred. No notable morphological change was found during the culture. K562 cells cultured in medium with CI grow slowly than that without CI. Conclusion： B7 costimulatory molecules expression on chronic myeloid leukemia cells line K562 surface was defective. These costimulatory molecules on K562 cells can be upregulated by calcium ionorphore. Calcium ionorphore may inhibit the growth of K562 cells.

关 键 词：钙离子载体 K562细胞 B7分子 共刺激分子 

分 类 号：R392.12[医药卫生—免疫学]