Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon  

作　　者：LIU Bin1,2,3,4, WANG Kemin1,2,3,4, XIAO Zhiqiang5,6, WANG Wei1,3,4, TAN Weihong1,2,3,4, SUN Yi5,6, TANG Hongxing1,3,4 & YANG Xiaohai1,3,41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan University, Changsha 410082, China 2. Life Science and Biotechnology Institute, Hunan University, Chang- sha 410082, China 3. Chemistry and Chemical Engineer College, Hunan University, Changsha 410082, China 4. Hunan Engineering Research Center for Bio-Nanotechnology, Hunan University, Changsha 410082, China 5. Medical Experiment Research Center, Xiangya Hospital, Central South University, Changsha 410008, China 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Changsha 410008, China 

出　　处：《Chinese Science Bulletin》2006年第17期2059-2064,共6页

基　　金：Acknowledgements This work was supported by the National Key Basic Research Program of China （Grant Nos. 2002CB 513110 and 2004CB520804）; the Key Technologies Research and Development Program （Grant No. 2003 BA310A 16）; the International Key Corporation Project of the Chinese Technology Ministry （Grant No. 2003DF000039）; the National Natural Science Foundation of China （Grant No. 20135010）; the Key Project of Natural Science Foundation of Hunan Province （Grant No. 0399Y1006）.

摘　　要：The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.The effects of drug treatment, gene transfection, and p53 RNA interference （RNAi） on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon （MB）/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10^-16 to 53.4× 10^-16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigating the interaction of multigenes on signal transduction pathway.

关 键 词：ING1 RT-PCR 定量分析 分子信号 MRNA 

分 类 号：Q75[生物学—分子生物学]