儿童福氏志贺菌Ⅰ类整合子的检测及其与耐药性的关系  

Detection of class Ⅰ integron and its relation to antimicrobial resistance of Shigella flexneri in children

作　　者：俞蕙[1] 王晓红[1] 叶颖子[1] 薛健昌[2] 朱启镕[1] 

机构地区：[1]复旦大学附属儿科医院传染科,上海200032 [2]复旦大学附属儿科医院细菌室,上海200032

出　　处：《中华儿科杂志》2006年第9期680-683,共4页Chinese Journal of Pediatrics

摘　　要：目的了解儿童福氏志贺菌是否存在Ⅰ类整合子及其发生率以及与耐药性的关系。方法对2004年6-11月从本院细菌性腹泻患儿大便培养标本分离到的福氏志贺菌共51株，应用PCR检测Ⅰ类整合子的5′保守区intⅠ1、基因盒区ant（3″）-Ⅰ和3′保守区qacE△1-sull；用K-B（Kriby-Bauer）法测定志贺菌对7种抗菌药物的敏感性。结果51株福氏志贺菌中46株检出intⅠ1，Ⅰ类整合子阳性率90．2％；24株检出qacE△1-sull，阳性率47．1％；intⅠ1、ant（3″）-Ⅰ和qacE△1-sull区段均阳性的为22株，阳性率43．1％；46株intⅠ1阳性福氏志贺菌ant（3″）-Ⅰ基因均阳性；46株intⅠ1阳性福氏志贺菌中qac△1-sull同时阳性的菌株为22株，占47．8％（22／46）、qacE△1-sull阴性的菌株24株，占52．2％（24／46）。Ⅰ类整合子阳性志贺菌对多种抗生素耐药，尤其是对氨苄西林（χ^2=10．13，P〈0．01）和氯霉素（χ^2=19．97，P〈0．01）的耐药性明显高于Ⅰ类整合子阴性志贺菌。结论从福氏志贺菌检出Ⅰ类整合子，携带率为90．2％；携带Ⅰ类整合子阳性志贺菌与抗生素耐药相关。Objective To investigate class Ⅰ integron of Shigella flexneri, its prevalence in children, and its relation to bacterial resistance to antimicrobial agents. Methods Totally 51 strains of Shigella flexneri were isolated from fecal samples of children suffering from bacterial diarrhea seen between June 2004 and November 2004 at Children＇s Hospital of Fudan University. Polymerase chain reaction （PCR） was employed to amplify various integron markers, including intⅠ1, gene cassette region and 3′ conserved region of class Ⅰ intrgron ; susceptibility of Shigella flexneri strains to 7 antimicrobial agents was determined by K-B （Kriby-Bauer） method. Results Forty-six strains of Shigellaflexneri had intI gene with a positive rate of 90. 2% （46/51 ） ; 24 strains of Shigellaflexneri were positive for qacE△1-sull, the positive rate was 47. 1% （ 24/51 ） ; proportion of the isolates positive for all the three regions of class Ⅰ integron was 43. 1% （22/51 ） ; 46 strains of intⅠ positive Shigella flexneri were all positive for ant （ 3″ ） -Ⅰ. Among 46 strains of intⅠ positive isolates, proportions of the isolates positive and negative for qacE△1 -sull were 47.8% （22/46） and 52.2% （24/46） , respectively. In the class Ⅰ integron positive ShigeUaflexneri, the resistance rates of ampicillin （χ^2 = 10. 13 ,P 〈0. 01 ） and chloramphenicol （χ^2 = 19. 97 ,P 〈0. 01 ） were significantly higher than those in the class Ⅰ integron-negative group. Conclusions Class I integron was detected in 90. 2% of Shigella flexrteri in children; carriage of class Ⅰ integron is related to antimicrobial resistance of Shigella flexneri.

关 键 词：志贺菌 弗氏 整合子类 抗药性 细菌 儿童 

分 类 号：R725.1[医药卫生—儿科]