应用上转磷光免疫层析技术快速定量检测炭疽芽孢  

Development of up-converting phosphor immunochromatography for fast and quantitive detection of Bacillus anthracis spore

作　　者：李伟[1] 周蕾[2] 王静[1] 胡孔新[1] 王津[2] 姚李四[1] 陈维娜[1] 闫中强[2] 牛春莉[1] 杨瑞馥[2] 

机构地区：[1]中国检验检疫科学研究院,北京100025 [2]微生物流行病研究所

出　　处：《中华微生物学和免疫学杂志》2006年第8期761-764,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家质检总局重大科研专项资助(2005IK0143-2)

摘　　要：目的建立上转磷光免疫层析技术快速定量检测炭疽芽孢。方法利用上转磷光检测技术和免疫层析技术(双抗体夹心法),建立检测炭疽芽孢的快速检测方法,对该方法的特异性、敏感性进行评价,在面粉、淀粉等材料中掺入炭疽芽孢模拟“白色粉末”,评价该法检测炭疽芽孢生物恐怖和环境样品的可行性,拟合检测曲线进行定量研究。结果该法可在30 min内完成定性检测,多种芽孢菌及其他病原菌评价显示该法特异性良好,检测灵敏性为104芽孢,模拟“白色粉末”的样品检测敏感性也相同,盲法考核,该法定性、定量结果较好。结论建立的检测炭疽芽孢杆菌的上转磷光免疫层析方法,能快速、特异、灵敏地检测炭疽芽孢,适用于现场快速检测。Objective To develop a method for fast and quantitive detection of anthrax spore. Methods Using up-converting phosphor technology based on double-antibody sandwich assay for the detection of B. anthracis spores. The sensitivity and specificity of the method were evaluated. The feasibility of screening environmental samples was evaluated by different ＂white powder＂ samples. Results The specificity of the assay was confirmed using closely related bacterial spores of B. cereus, B. thuringiensis, B. subtilis and of other bacteria. The sensitivity is 104 spores and the same results were obtained from ＂white powder＂ samples. Conclusion The immunochromatography based on up-converting phosphor technology provides a fast, specific and sensitive method for the detection of B. anthacis spore on site. It is potentially suited to identify B. anthracis spores.

关 键 词：炭疽芽孢杆菌 上转磷光技术 免疫层析法 

分 类 号：R446.6[医药卫生—诊断学]