环氧化酶-2在闭合性颅脑损伤中的表达  

Expression of cyclooxygenase-2 gene in rats with traumatic brain injury

作　　者：李志强[1] 张永亮[2] 李灵芝[3] 舒清明[4] 

机构地区：[1]武警内蒙古总队医院内二科,呼和浩特010031 [2]武警医学院研究生处,天津300162 [3]武警医学院药化教研室,天津300162 [4]武警总医院病理科,北京100039

出　　处：《武警医学》2006年第8期570-573,F0003,共5页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金项目(项目编号30471934)

摘　　要：目的研究闭合性脑损伤后COX-2在脑组织中的表达部位、时序性特点。方法采用免疫组化SP法检测SD大鼠脑组织COX-2的表达情况。结果损伤后，顶叶皮质、CA4、CA3、脑底皮质阳性细胞面积、阳性细胞计数与对照组比较差别有统计学意义（P〈0．01）。结论脑损伤后，COX-2表达在1．2h开始增加，8．12h达最高值，5．7d时基本恢复正常.Objective To study the expression site and the temporal patterns ot cyclooxygenase- 2（COX- 2） in rats with closed traumatic brain injury （TBI） .Methods The expression of COX- 2 was studied by immunohistochemistry in the brain of Sprague - Dawley rats. Results After brain injury, the number and area of COX- 2 positive neurons increased progressively in parietal cortex, hippocampns CA4, CA3, basicmnial cortex compared with sham operatied controls （ P 〈 0.01 ）. Conclusions After closed traumatic brain injury, for 1 - 2 h, the expression of COX - 2 in brain tissue increases progressively, and reaches to the peak level after 8 - 12 h, and returns to the normal after 5 - 7d.

关 键 词：闭合性颅脑损伤 环氧化酶-2 

分 类 号：R651.15[医药卫生—外科学]