薄层色谱-荧光分析法测定水罗伞药材中水黄皮素含量的研究  

Determination of Karanjin in Radix of Fofdia Caulifora by Thin Layer Chromatograph- Fluorescent Method

作　　者：杨东爱[1] 杨立芳[2] 马少妹[2] 尹显洪[2] 戴斌[1] 

机构地区：[1]广西民族医药研究所,广西南宁530001 [2]广西民族学院,广西南宁530000

出　　处：《中国中医药信息杂志》2006年第9期53-54,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：广西自然科学基金(0447016)

摘　　要：目的建立薄层色谱-荧光分光光度法测定水罗伞中水黄皮素含量的方法。方法采用无水乙醇为溶剂,硅胶G为薄层板,苯-乙酸乙酯(8.5∶1.5)为展开剂,激发波长与荧光波长分别为355nm和428nm。结果水黄皮素线性范围为0.138～0.69μg,r=0.9991,平均回收率为99.14%,RSD为1.94%(n=5)。结论该法便捷、灵敏、准确、重现性好,可以控制该药材的质量。Objective To establish a thin layer Chromatograph-Fluores cent method for the determination 4 of karanjin in Radix of Fofdia caulifora. Methods Anhydrous-EtOH is solution, thin-sheet is silica gel, expanding solution is Benzene-EtoAc （8.5 ：1.5）, with excitation wavelength at 355 nm and emission wavelength at 428 nm. Results The average recovery of karanjin was 99.14% and RSD was 1.94% （n=5）. There is a good linear relationship within the range of 0. 138-0.69 μg of karanjin. Conclusion The method is convenient, sensitive, accurate and reproducible, and can be used the quality control of the Radix of Fofdia caulffora.

关 键 词：水罗伞 水黄皮素 薄层色谱-荧光光谱法 

分 类 号：R284.1[医药卫生—中药学]